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Isolation and identification of Bacillus megaterium YB3 from an effluent contaminated site efficiently degrades pyrene.
- Source :
-
Journal of basic microbiology [J Basic Microbiol] 2016 Apr; Vol. 56 (4), pp. 369-78. Date of Electronic Publication: 2016 Jan 11. - Publication Year :
- 2016
-
Abstract
- Industrial effluents contaminated sites may serve as repositories of ecologically adapted efficient pyrene degrading bacteria. In the present study, six bacterial isolates from industrial effluents were purified using serial enrichment technique and their pyrene degrading potential on pyrene supplemented mineral salt medium was assessed. 16S rRNA sequence analysis showed that they belong to four bacterial genera, namely Acinetobacter, Bacillus, Microbacterium, and Ochrobactrum. Among these isolates, Bacillus megaterium YB3 showed considerably good growth and was further evaluated for its pyrene-degrading efficiency. B. megaterium YB3 could degrade 72.44% of 500 mg L(-1) pyrene within 7 days. GC-MS analysis of ethyl acetate extracted fractions detected two relatively less toxic metabolic intermediates of the pyrene degradation pathway. B. megaterium YB3 also tested positive for catechol 1, 2-dioxygenase and aromatic-ring-hydroxylating dioxygenase indole-indigo conversion assays. Considering the ability and efficiency of B. megaterium YB3 to degrade high pyrene content, the strain can be used as a tool to develop bioremediation technologies for the effective biodegradation of pyrene and possibly other PAHs in the environment.<br /> (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Acetates chemistry
Bacillus megaterium enzymology
Bacillus megaterium genetics
Biodegradation, Environmental
Catechol 1,2-Dioxygenase analysis
Dioxygenases analysis
Enzyme Activation
Indigo Carmine metabolism
Polycyclic Aromatic Hydrocarbons metabolism
Pyrenes chemistry
RNA, Ribosomal, 16S genetics
Bacillus megaterium isolation & purification
Bacillus megaterium metabolism
Pyrenes metabolism
Soil Microbiology
Soil Pollutants metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1521-4028
- Volume :
- 56
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of basic microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 26755240
- Full Text :
- https://doi.org/10.1002/jobm.201500533