Back to Search
Start Over
Over-expression of Arabidopsis thaliana SFD1/GLY1, the gene encoding plastid localized glycerol-3-phosphate dehydrogenase, increases plastidic lipid content in transgenic rice plants.
- Source :
-
Journal of plant research [J Plant Res] 2016 Mar; Vol. 129 (2), pp. 285-293. Date of Electronic Publication: 2016 Jan 08. - Publication Year :
- 2016
-
Abstract
- Lipids are the major constituents of all membranous structures in plants. Plants possess two pathways for lipid biosynthesis: the prokaryotic pathway (i.e., plastidic pathway) and the eukaryotic pathway (i.e., endoplasmic-reticulum (ER) pathway). Whereas some plants synthesize galactolipids from diacylglycerol assembled in the plastid, others, including rice, derive their galactolipids from diacylglycerols assembled by the eukaryotic pathway. Arabidopsis thaliana glycerol-3-phosphate dehydrogenase (G3pDH), coded by SUPPRESSOR OF FATTY ACID DESATURASE 1 (SFD1; alias GLY1) gene, catalyzes the formation of glycerol 3-phosphate (G3p), the backbone of many membrane lipids. Here SFD1 was introduced to rice as a transgene. Arabidopsis SFD1 localizes in rice plastids and its over-expression increases plastidic membrane lipid content in transgenic rice plants without any major impact on ER lipids. The results suggest that over-expression of plastidic G3pDH enhances biosynthesis of plastid-localized lipids in rice. Lipid composition in the transgenic plants is consistent with increased phosphatidylglycerol synthesis in the plastid and increased galactolipid synthesis from diacylglycerol produced via the ER pathway. The transgenic plants show a higher photosynthetic assimilation rate, suggesting a possible application of this finding in crop improvement.
- Subjects :
- Arabidopsis cytology
Arabidopsis genetics
Arabidopsis Proteins metabolism
Fatty Acids analysis
Fatty Acids metabolism
Gene Expression
Gene Expression Regulation, Plant
Genes, Reporter
Glycerolphosphate Dehydrogenase metabolism
Lipid Metabolism
Oryza genetics
Photosynthesis
Plants, Genetically Modified
Plastids enzymology
Recombinant Fusion Proteins
Seedlings cytology
Seedlings enzymology
Seedlings genetics
Arabidopsis enzymology
Arabidopsis Proteins genetics
Galactolipids metabolism
Glycerolphosphate Dehydrogenase genetics
Glycerophosphates metabolism
Lipids analysis
Oryza metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0860
- Volume :
- 129
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of plant research
- Publication Type :
- Academic Journal
- Accession number :
- 26747130
- Full Text :
- https://doi.org/10.1007/s10265-015-0781-0