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Evaluation of intraoperative fluorescence imaging-guided surgery in cancer-bearing dogs: a prospective proof-of-concept phase II study in 9 cases.
Evaluation of intraoperative fluorescence imaging-guided surgery in cancer-bearing dogs: a prospective proof-of-concept phase II study in 9 cases.
- Source :
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Translational research : the journal of laboratory and clinical medicine [Transl Res] 2016 Apr; Vol. 170, pp. 73-88. Date of Electronic Publication: 2015 Dec 19. - Publication Year :
- 2016
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Abstract
- The objective was to prospectively evaluate the application of intraoperative fluorescence imaging (IOFI) in the surgical excision of malignant masses in dogs, using a novel lipid nanoparticle contrast agent. Dogs presenting with spontaneous soft-tissue sarcoma or subcutaneous tumors were prospectively enrolled. Clinical staging and whole-body computed tomography (CT) were performed. All the dogs received an intravenous injection of dye-loaded lipid nanoparticles, LipImage 815. Wide or radical resection was realized after CT examination. Real-time IOFI was performed before skin incision and after tumor excision. In cases of radical resection, the lymph nodes (LNs) were imaged. The margin/healthy tissues fluorescence ratio or LN/healthy tissues fluorescence ratio was measured and compared with the histologic margins or LN status. Nine dogs were included. Limb amputation was performed in 3 dogs, and wide resection in 6. No adverse effect was noted. Fluorescence was observed in all 9 of the tumors. The margins were clean in 5 of 6 dogs after wide surgical resection, and the margin/healthy tissues fluorescence ratio was close to 1.0 in all these dogs. Infiltrated margins were observed in 1 case, with a margin/healthy tissues fluorescence ratio of 3.2. Metastasis was confirmed in 2 of 3 LNs, associated with LN/healthy tissues fluorescence ratios of 2.1 and 4.2, whereas nonmetastatic LN was associated with a ratio of 1.0. LipImage 815 used as a contrast agent during IOFI seemed to allow for good discrimination between tumoral and healthy tissues. Future studies are scheduled to evaluate the sensitivity and specificity of IOFI using LipImage 815 as a tracer.<br /> (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Contrast Media administration & dosage
Dogs
Female
Fluorescence
Fluorescent Dyes administration & dosage
Indoles administration & dosage
Intraoperative Care methods
Lymphatic Metastasis pathology
Male
Nanoparticles administration & dosage
Prospective Studies
Sarcoma pathology
Sarcoma surgery
Sarcoma veterinary
Soft Tissue Neoplasms pathology
Soft Tissue Neoplasms surgery
Spectroscopy, Near-Infrared methods
Tomography, X-Ray Computed
Dog Diseases surgery
Image Processing, Computer-Assisted methods
Soft Tissue Neoplasms veterinary
Surgery, Computer-Assisted methods
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1810
- Volume :
- 170
- Database :
- MEDLINE
- Journal :
- Translational research : the journal of laboratory and clinical medicine
- Publication Type :
- Academic Journal
- Accession number :
- 26746803
- Full Text :
- https://doi.org/10.1016/j.trsl.2015.12.001