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The PB1 segment of an influenza A virus H1N1 2009pdm isolate enhances the replication efficiency of specific influenza vaccine strains in cell culture and embryonated eggs.
- Source :
-
The Journal of general virology [J Gen Virol] 2016 Mar; Vol. 97 (3), pp. 620-631. Date of Electronic Publication: 2016 Jan 07. - Publication Year :
- 2016
-
Abstract
- Influenza vaccine strains (IVSs) contain the haemagglutinin (HA) and neuraminidase (NA) genome segments of relevant circulating strains in the genetic background of influenza A/PR/8/1934 virus (PR8). Previous work has shown that the nature of the PB1 segment may be a limiting factor for the efficient production of IVSs. Here, we showed that the PB1 segment (PB1Gi) from the 2009 pandemic influenza A virus (IAV) A/Giessen/06/2009 (Gi wt, H1N1pdm) may help to resolve (some of) these limitations. We produced a set of recombinant PR8-derived viruses that contained (i) the HA and NA segments from representative IAV strains (H3N2, H5N1, H7N9, H9N2); (ii) the PB1 segment from PR8 or Gi wt, respectively; and (iii) the remaining five genome segments from PR8. Viruses containing the PB1Gi segment, together with the heterologous HA/NA segments and five PR8 segments (5+2+1), replicated to higher titres compared with their 6+2 counterparts containing six PR8 segments and the equivalent heterologous HA/NA segments. Compared with PB1PR8-containing IVSs, viruses with the PB1Gi segment replicated to higher or similar titres in both cell culture and embryonated eggs, most profoundly IVSs of the H5N1 and H7N9 subtype, which are known to grow poorly in these systems. IVSs containing either the PB1Gi or the cognate PB1 segment of the respective specific HA/NA donor strain showed enhanced or similar virus replication levels. This study suggests that substitution of PB1PR8 with the PB1Gi segment may greatly improve the large-scale production of PR8-derived IVSs, especially of those known to replicate poorly in vitro.
- Subjects :
- Animals
Chick Embryo
Hemagglutinin Glycoproteins, Influenza Virus genetics
Hemagglutinin Glycoproteins, Influenza Virus metabolism
Humans
Influenza A Virus, H1N1 Subtype genetics
Influenza A Virus, H1N1 Subtype immunology
Influenza A Virus, H1N1 Subtype physiology
Influenza A Virus, H3N2 Subtype genetics
Influenza A Virus, H3N2 Subtype physiology
Influenza A Virus, H5N1 Subtype genetics
Influenza A Virus, H5N1 Subtype physiology
Influenza A Virus, H7N9 Subtype genetics
Influenza A Virus, H7N9 Subtype physiology
Influenza A Virus, H9N2 Subtype genetics
Influenza A Virus, H9N2 Subtype physiology
Influenza Vaccines genetics
Influenza Vaccines immunology
Influenza, Human epidemiology
Influenza, Human prevention & control
Ovum virology
Viral Proteins genetics
Influenza A Virus, H1N1 Subtype enzymology
Influenza Vaccines administration & dosage
Influenza, Human virology
Viral Proteins metabolism
Virus Replication
Subjects
Details
- Language :
- English
- ISSN :
- 1465-2099
- Volume :
- 97
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 26743314
- Full Text :
- https://doi.org/10.1099/jgv.0.000390