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Pseudoclavibacter endophyticus sp. nov., isolated from roots of Glycyrrhiza uralensis .

Authors :
Li YQ
Li L
Fu YS
Cui ZQ
Duan YQ
Salam N
Guo JW
Chen W
Li WJ
Source :
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2016 Mar; Vol. 66 (3), pp. 1287-1292. Date of Electronic Publication: 2016 Jan 05.
Publication Year :
2016

Abstract

A Gram-stain-positive, aerobic, rod-shaped, non-motile actinomycete strain, designated EGI 60007 <superscript>T</superscript> , was isolated from healthy roots of Glycyrrhiza uralensis F. collected from Yili County, Xinjiang Province, north-west China. A polyphasic approach was applied to study the taxonomic position of the new isolate. The 16S rRNA gene sequence of strain EGI 60007 <superscript>T</superscript> had highest similarities with members of the genus Pseudoclavibacter , including Pseudoclavibacter chungangensis CAU 59 <superscript>T</superscript> (96.98 % 16S rRNA gene sequence similarity), Pseudoclavibacter helvolus DSM 20419 <superscript>T</superscript> (96.43 %) and Pseudoclavibacter terrae THG-MD12 <superscript>T</superscript> (96.14 %). The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain EGI 60007 <superscript>T</superscript> clustered with members of the genus Pseudoclavibacter , and formed a distinct clade with P. chungangensis CAU 59 <superscript>T</superscript> . The polar lipids detected for strain EGI 60007 <superscript>T</superscript> were phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified lipid. The DNA G+C content was determined to be 63.3 mol%. The chemotaxonomic features of strain EGI 60007 <superscript>T</superscript> showed typical characteristics of the genus Pseudoclavibacter , with MK-9 as the respiratory quinone, 2,4-diaminobutyric acid as the diamino acid in the peptidoglycan, and anteiso-C <subscript>15 : 0</subscript> , anteiso-C <subscript>17 : 0</subscript> and iso-C <subscript>16 : 0</subscript> as the major fatty acids. The sugars of whole-cell hydrolysates were mainly mannose, rhamnose, ribose and glucose, and a minor amount of xylose. Based on the results of the phylogentic analysis supported by morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60007 <superscript>T</superscript> is considered to represent a novel species of the genus Pseudoclavibacter , for which the name Pseudoclavibacter endophyticus sp. nov. is proposed. The type strain is EGI 60007 <superscript>T</superscript> ( = CGMCC 1.15081 <superscript>T</superscript> = KCTC 39112 <superscript>T</superscript> = DSM 29943 <superscript>T</superscript> ).

Details

Language :
English
ISSN :
1466-5034
Volume :
66
Issue :
3
Database :
MEDLINE
Journal :
International journal of systematic and evolutionary microbiology
Publication Type :
Academic Journal
Accession number :
26739161
Full Text :
https://doi.org/10.1099/ijsem.0.000876