Back to Search
Start Over
Rhinovirus inhibits IL-17A and the downstream immune responses in allergic asthma.
- Source :
-
Mucosal immunology [Mucosal Immunol] 2016 Sep; Vol. 9 (5), pp. 1183-92. Date of Electronic Publication: 2016 Jan 06. - Publication Year :
- 2016
-
Abstract
- The proinflammatory cytokine interleukin-17A (IL-17A) is known to mediate antimicrobial activity, but its role during rhinovirus (RV) infections and in asthma needs further investigation. Therefore, we addressed the role of IL-17A during allergic asthma and antiviral immune response in human and murine immunocompetent cells. In this study we found that asthmatic children with a RV infection in their upper airways have upregulated mRNA levels of the antiviral cytokine interferon type I (IFN)-β and the transcription factor T-box 21 (TBX21) and reduced levels of IL-17A protein in their peripheral blood mononuclear cells (PBMCs). We also found that IL-17A inhibited RV1b replication in infected human lung epithelial cells A549. Furthermore, by using gene array analysis we discovered that targeted deletion of Il17a in murine lung CD4(+) T cells impaired Oas1g mRNA downstream of Ifnβ, independently from RV infection. Additionally, in PBMCs of children with a RV infection in their nasalpharyngeal fluid OAS1 gene expression was found downregulated. Finally RV1b inhibited IL-17A production in lung CD4(+) T cells in a setting of experimental asthma. These results indicate that the RV1b inhibits IL-17A in T helper type 17 cells and IL-17A clears RV1b infection in epithelial cells. In both cases IL-17A contributes to fend off RV1b infection by inducing genes downstream of interferon type I pathway.
- Subjects :
- 2',5'-Oligoadenylate Synthetase genetics
2',5'-Oligoadenylate Synthetase immunology
A549 Cells
Animals
Asthma genetics
Asthma virology
CD4-Positive T-Lymphocytes virology
Child
Child, Preschool
Drug Hypersensitivity genetics
Drug Hypersensitivity virology
Female
Gene Expression Regulation
Humans
Interferon-beta genetics
Interferon-beta immunology
Interleukin-17 genetics
Lung immunology
Lung virology
Male
Mice
Mice, Knockout
Ovalbumin administration & dosage
Picornaviridae Infections genetics
Picornaviridae Infections virology
Primary Cell Culture
RNA, Messenger genetics
RNA, Messenger immunology
Rhinovirus growth & development
Signal Transduction
T-Box Domain Proteins genetics
T-Box Domain Proteins immunology
Asthma immunology
CD4-Positive T-Lymphocytes immunology
Drug Hypersensitivity immunology
Interleukin-17 immunology
Picornaviridae Infections immunology
Rhinovirus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1935-3456
- Volume :
- 9
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Mucosal immunology
- Publication Type :
- Academic Journal
- Accession number :
- 26732679
- Full Text :
- https://doi.org/10.1038/mi.2015.130