Back to Search
Start Over
Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue.
- Source :
-
Cell reports [Cell Rep] 2016 Jan 05; Vol. 14 (1), pp. 152-167. Date of Electronic Publication: 2015 Dec 24. - Publication Year :
- 2016
-
Abstract
- E-cadherin-mediated cell-cell junctions play a prominent role in maintaining the epithelial architecture. The disruption or deregulation of these adhesions in cancer can lead to the collapse of tumor epithelia that precedes invasion and subsequent metastasis. Here we generated an E-cadherin-GFP mouse that enables intravital photobleaching and quantification of E-cadherin mobility in live tissue without affecting normal biology. We demonstrate the broad applications of this mouse by examining E-cadherin regulation in multiple tissues, including mammary, brain, liver, and kidney tissue, while specifically monitoring E-cadherin mobility during disease progression in the pancreas. We assess E-cadherin stability in native pancreatic tissue upon genetic manipulation involving Kras and p53 or in response to anti-invasive drug treatment and gain insights into the dynamic remodeling of E-cadherin during in situ cancer progression. FRAP in the E-cadherin-GFP mouse, therefore, promises to be a valuable tool to fundamentally expand our understanding of E-cadherin-mediated events in native microenvironments.<br /> (Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cadherins genetics
Green Fluorescent Proteins genetics
Mice
Mice, Transgenic
Neoplasms, Experimental genetics
Organ Specificity
Proto-Oncogene Proteins p21(ras) genetics
Proto-Oncogene Proteins p21(ras) metabolism
Tumor Suppressor Protein p53 genetics
Tumor Suppressor Protein p53 metabolism
Cadherins metabolism
Green Fluorescent Proteins metabolism
Neoplasms, Experimental metabolism
Neoplasms, Experimental pathology
Optical Imaging methods
Tumor Microenvironment
Subjects
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 26725115
- Full Text :
- https://doi.org/10.1016/j.celrep.2015.12.020