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Effects of cryotherapy combined with therapeutic ultrasound on oxidative stress and tissue damage after musculoskeletal contusion in rats.

Effects of cryotherapy combined with therapeutic ultrasound on oxidative stress and tissue damage after musculoskeletal contusion in rats.

Authors :
Martins CN
Moraes MB
Hauck M
Guerreiro LF
Rossato DD
Varela AS Jr
da Rosa CE
Signori LU
Source :
Physiotherapy [Physiotherapy] 2016 Dec; Vol. 102 (4), pp. 377-383. Date of Electronic Publication: 2015 Nov 29.
Publication Year :
2016

Abstract

Objective: To investigate the combined effects of cryotherapy and pulsed ultrasound therapy (PUT) on oxidative stress parameters, tissue damage markers and systemic inflammation after musculoskeletal injury.<br />Design: Experimental animal study.<br />Setting: Research laboratory.<br />Participants: Seventy male Wistar rats were divided into five groups: control, lesion, cryotherapy, PUT, and cryotherapy+PUT.<br />Interventions: The gastrocnemius muscle was injured by mechanical crushing. Cryotherapy was applied immediately after injury (immersion in water at 10°C for 20minutes). PUT was commenced 24hours after injury (1MHz, 0.4W/cm <superscript>2SPTA</superscript> , 20% duty cycle, 5minutes). All animals were treated every 8hours for 3 days.<br />Main Outcome Measures: Oxidative stress in muscle was evaluated by concentration of reactive oxygen species (ROS), lipid peroxidation (LPO), anti-oxidant capacity against peroxyl radicals (ACAP) and catalase. Plasma levels of creatine kinase (CK), lactate dehydrogenase (LDH) and C-reactive protein (CRP) were assessed.<br />Results: When applied individually, cryotherapy and PUT reduced CK, LDH, CRP and LPO caused by muscle damage. Cryotherapy+PUT in combination maintained the previous results, caused a reduction in ROS [P=0.005, mean difference -0.9×10 <superscript>-8</superscript> relative area, 95% confidence interval (CI) -0.2 to -1.9], and increased ACAP {P=0.007, mean difference 0.34 1/[relative area with/without 2,2-azobis(2-methylpropionamidine)dihydrochloride], 95% CI 0.07 to 0.61} and catalase (P=0.002, mean difference 0.41units/mg protein, 95% CI 0.09 to 0.73) compared with the lesion group.<br />Conclusions: Cryotherapy+PUT in combination reduced oxidative stress in muscle, contributing to a reduction in adjacent damage and tissue repair.<br /> (Copyright © 2016 Chartered Society of Physiotherapy. Published by Elsevier Ltd. All rights reserved.)

Details

Language :
English
ISSN :
1873-1465
Volume :
102
Issue :
4
Database :
MEDLINE
Journal :
Physiotherapy
Publication Type :
Academic Journal
Accession number :
26725004
Full Text :
https://doi.org/10.1016/j.physio.2015.10.013