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Genetic characterization and evolutionary analysis of Newcastle disease virus isolated from domestic duck in South Korea.
- Source :
-
Gene [Gene] 2016 Mar 15; Vol. 579 (1), pp. 34-40. Date of Electronic Publication: 2015 Dec 22. - Publication Year :
- 2016
-
Abstract
- Domestic ducks are considered a potential reservoir of Newcastle disease virus. In the study, a Newcastle disease virus (NDV) isolated from a domestic duck during surveillance in South Korea was characterized. The complete genome of the NDV isolate was sequenced, and the phylogenetic relationship to reference strains was studied. Phylogenetic analysis revealed that the strain clustered in genotype I of Class II ND viruses, has highly phylogenetic similarity to NDV strains isolated from waterfowl in China, but was distant from the viruses isolated in chickens and vaccine strains used in South Korea. Pathogenicity experiment in chickens revealed it to be a lentogenic virus. The deduced amino acid sequence of the cleavage site of the fusion (F) protein confirmed that the isolate contained the avirulent motif (112)GKQGRL(117) at the cleavage site and caused no apparent disease in chickens and ducks. With phylogeographic analysis based on fusion gene, we estimate the origin of an ancestral virus of the isolate and its sister strain located in China around 1998. It highlights the need of continuous surveillance to enhance current understanding of the molecular epidemiology and evolution of the pathogenic strains.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
China
Evolution, Molecular
Genome, Viral
Newcastle disease virus pathogenicity
Phylogeny
Phylogeography
RNA, Viral analysis
Republic of Korea
Sequence Analysis, RNA
Ducks
Newcastle Disease virology
Newcastle disease virus genetics
Newcastle disease virus isolation & purification
Poultry Diseases virology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0038
- Volume :
- 579
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 26721461
- Full Text :
- https://doi.org/10.1016/j.gene.2015.12.040