Back to Search Start Over

Genetic characterization and evolutionary analysis of Newcastle disease virus isolated from domestic duck in South Korea.

Authors :
Gaikwad S
Kim JY
Lee HJ
Jung SC
Choi KS
Source :
Gene [Gene] 2016 Mar 15; Vol. 579 (1), pp. 34-40. Date of Electronic Publication: 2015 Dec 22.
Publication Year :
2016

Abstract

Domestic ducks are considered a potential reservoir of Newcastle disease virus. In the study, a Newcastle disease virus (NDV) isolated from a domestic duck during surveillance in South Korea was characterized. The complete genome of the NDV isolate was sequenced, and the phylogenetic relationship to reference strains was studied. Phylogenetic analysis revealed that the strain clustered in genotype I of Class II ND viruses, has highly phylogenetic similarity to NDV strains isolated from waterfowl in China, but was distant from the viruses isolated in chickens and vaccine strains used in South Korea. Pathogenicity experiment in chickens revealed it to be a lentogenic virus. The deduced amino acid sequence of the cleavage site of the fusion (F) protein confirmed that the isolate contained the avirulent motif (112)GKQGRL(117) at the cleavage site and caused no apparent disease in chickens and ducks. With phylogeographic analysis based on fusion gene, we estimate the origin of an ancestral virus of the isolate and its sister strain located in China around 1998. It highlights the need of continuous surveillance to enhance current understanding of the molecular epidemiology and evolution of the pathogenic strains.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1879-0038
Volume :
579
Issue :
1
Database :
MEDLINE
Journal :
Gene
Publication Type :
Academic Journal
Accession number :
26721461
Full Text :
https://doi.org/10.1016/j.gene.2015.12.040