Analysis of Assembly and Budding of Lujo Virus.

Authors :: Urata S
Weyer J
Storm N
Miyazaki Y
van Vuren PJ
Paweska JT
Yasuda J
Source :: Journal of virology [J Virol] 2015 Dec 30; Vol. 90 (6), pp. 3257-61. Date of Electronic Publication: 2015 Dec 30.
Publication Year :: 2015
Abstract: The recently identified arenavirus Lujo virus (LUJV) causes fatal hemorrhagic fever in humans. We analyzed its mechanism of viral release driven by matrix protein Z and the cell surface glycoprotein precursor GPC. The L domains in Z are required for efficient virus-like particle release, but Tsg101, ALIX/AIP1, and Vps4A/B are unnecessary for budding. LUJV GPC is cleaved by site 1 protease (S1P) at the RKLM motif, and treatment with the S1P inhibitor PF-429242 reduced LUJV production.<br /> (Copyright © 2016, American Society for Microbiology. All Rights Reserved.)