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Resolvin D1 protects against inflammation in experimental acute pancreatitis and associated lung injury.
- Source :
-
American journal of physiology. Gastrointestinal and liver physiology [Am J Physiol Gastrointest Liver Physiol] 2016 Mar 01; Vol. 310 (5), pp. G303-9. Date of Electronic Publication: 2015 Dec 23. - Publication Year :
- 2016
-
Abstract
- Acute pancreatitis is an inflammatory condition that may lead to multisystemic organ failure with considerable mortality. Recently, resolvin D1 (RvD1) as an endogenous anti-inflammatory lipid mediator has been confirmed to protect against many inflammatory diseases. This study was designed to investigate the effects of RvD1 in acute pancreatitis and associated lung injury. Acute pancreatitis varying from mild to severe was induced by cerulein or cerulein combined with LPS, respectively. Mice were pretreated with RvD1 at a dose of 300 ng/mouse 30 min before the first injection of cerulein. Severity of AP was assessed by biochemical markers and histology. Serum cytokines and myeloperoxidase (MPO) levels in pancreas and lung were determined for assessing the extent of inflammatory response. NF-κB activation was determined by Western blotting. The injection of cerulein or cerulein combined with LPS resulted in local injury in the pancreas and corresponding systemic inflammatory changes with pronounced severity in the cerulein and LPS group. Pretreated RvD1 significantly reduced the degree of amylase, lipase, TNF-α, and IL-6 serum levels; the MPO activities in the pancreas and the lungs; the pancreatic NF-κB activation; and the severity of pancreatic injury and associated lung injury, especially in the severe acute pancreatitis model. These results suggest that RvD1 is capable of improving injury of pancreas and lung and exerting anti-inflammatory effects through the inhibition of NF-κB activation in experimental acute pancreatitis, with more notable protective effect in severe acute pancreatitis. These findings indicate that RvD1 may constitute a novel therapeutic strategy in the management of severe acute pancreatitis.<br /> (Copyright © 2016 the American Physiological Society.)
- Subjects :
- Animals
Anti-Inflammatory Agents metabolism
Anti-Inflammatory Agents pharmacology
Ceruletide pharmacology
Disease Models, Animal
Gastrointestinal Agents pharmacology
Interleukin-6 metabolism
Lung metabolism
Lung pathology
Mice
Mice, Inbred C57BL
NF-kappa B metabolism
Pancreas metabolism
Pancreas pathology
Peroxidase metabolism
Protective Agents metabolism
Protective Agents pharmacology
Signal Transduction drug effects
Docosahexaenoic Acids metabolism
Docosahexaenoic Acids pharmacology
Inflammation drug therapy
Inflammation metabolism
Lung Injury etiology
Lung Injury metabolism
Lung Injury pathology
Pancreatitis, Acute Necrotizing complications
Pancreatitis, Acute Necrotizing metabolism
Pancreatitis, Acute Necrotizing pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1547
- Volume :
- 310
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Gastrointestinal and liver physiology
- Publication Type :
- Academic Journal
- Accession number :
- 26702138
- Full Text :
- https://doi.org/10.1152/ajpgi.00355.2014