Back to Search
Start Over
Metabolomic profiles investigation on athletes' urine 35 minutes after an 800-meter race.
- Source :
-
The Journal of sports medicine and physical fitness [J Sports Med Phys Fitness] 2017 Jun; Vol. 57 (6), pp. 839-849. Date of Electronic Publication: 2015 Dec 23. - Publication Year :
- 2017
-
Abstract
- Background: The aim of this study was to identify possible biological pathways of the metabolite profile changes in athletes' urine samples before and after 800-m runs.<br />Methods: We used an NMR-based metabolomics analysis to evaluate the metabolite profile changes in 19 young male athletes' urine samples after 800-m runs and provide an overall picture of its impact. Various multivariate data analysis methods, including principal component analysis (PCA), partial least squares-discrimination analysis (PLS-DA), and orthogonal projection of latent-structure-discrimination analysis (OPLS-DA) were applied to analyze the NMR data and thus identify possible correlations between the metabolite profile changes and the alterations in biological pathways.<br />Results: The potential biological mechanism of an 800-m race was finally elucidated based on the multivariate statistical analysis results. The levels of blood lactate (Lac), 2-hydroxyisovalerate (2HIV), leucine, 2-hydroxyisobutyrate (2HIB), alanine, N-acetyl-glucoprotein, pyruvate, creatinine, fumarate, inosine (Ino) and hypoxanthine (Hyx) were up-regulated in the post samples, whereas the levels of certain metabolites, including 3-hydroxyisovalerate, citrate, taurine, glycine and formate were down-regulated in the postsamples.<br />Conclusions: Our study provides novel insights into the 800-m race metabolic characteristic. Separation of pre- from postexercise samples was related to the Krebs cycle, Cori cycle, Cahill cycle, HIFs and ROS. Besides the Lac change, the increased concentrations of Ino, 2HIV concentrations in the postexercise urine samples represent potential indices which indicate the high percent of glycolysis during the 800-m run. The increase of concentrations of Hyx, 2HIB may indicated oxidative stress with concomitant ROS generation in the athletes' bodies during the 800-m race.
- Subjects :
- Adolescent
Biomarkers urine
Creatinine urine
Formates urine
Heart Rate
Humans
Lactic Acid urine
Least-Squares Analysis
Magnetic Resonance Spectroscopy
Male
Oxidative Stress physiology
Principal Component Analysis
Time Factors
Young Adult
Hydroxybutyrates urine
Metabolomics methods
Running physiology
Valerates urine
Subjects
Details
- Language :
- English
- ISSN :
- 1827-1928
- Volume :
- 57
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of sports medicine and physical fitness
- Publication Type :
- Academic Journal
- Accession number :
- 26699119
- Full Text :
- https://doi.org/10.23736/S0022-4707.17.06254-5