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Preparation of Ca(2+)-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum.

Authors :
Møller JV
Olesen C
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2016; Vol. 1377, pp. 11-7.
Publication Year :
2016

Abstract

The SERCA calcium ATPase, probably the most well-investigated membrane protein both from a biophysical and structural view, can be purified from native source in substantial quantities, making it a favorable target when conducting biochemical experiments and structure determination, e.g., by X-ray crystallography.

Subjects

Subjects :
Animals
Calcium chemistry
Crystallography, X-Ray
Protein Conformation
Rabbits
Sarcoplasmic Reticulum Calcium-Transporting ATPases chemistry
Molecular Biology methods
Sarcoplasmic Reticulum enzymology
Sarcoplasmic Reticulum Calcium-Transporting ATPases isolation & purification

Details

Language :
English
ISSN :
1940-6029
Volume :
1377
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
26695018
Full Text :
https://doi.org/10.1007/978-1-4939-3179-8_3
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