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Characterization of the legumains encoded by the genome of Theobroma cacao L.
- Source :
-
Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2016 Jan; Vol. 98, pp. 162-70. Date of Electronic Publication: 2015 Dec 02. - Publication Year :
- 2016
-
Abstract
- Legumains are cysteine proteases related to plant development, protein degradation, programmed cell death, and defense against pathogens. In this study, we have identified and characterized three legumains encoded by Theobroma cacao genome through in silico analyses, three-dimensional modeling, genetic expression pattern in different tissues and as a response to the inoculation of Moniliophthora perniciosa fungus. The three proteins were named TcLEG3, TcLEG6, and TcLEG9. Histidine and cysteine residue which are part of the catalytic site were conserved among the proteins, and they remained parallel in the loop region in the 3D modeling. Three-dimensional modeling showed that the propeptide, which is located in the terminal C region of legumains blocks the catalytic cleft. Comparing dendrogram data with the relative expression analysis, indicated that TcLEG3 is related to the seed legumain group, TcLEG6 is related with the group of embryogenesis activities, and protein TcLEG9, with processes regarding the vegetative group. Furthermore, the expression analyses proposes a significant role for the three legumains during the development of Theobroma cacao and in its interaction with M. perniciosa.<br /> (Copyright © 2015 Universidade Estadual de Santa Cruz, CNPJ: 40738999/0001-95. Published by Elsevier Masson SAS.. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Cacao genetics
Cacao growth & development
Cacao immunology
Cluster Analysis
Cotyledon enzymology
Cotyledon genetics
Cotyledon growth & development
Cotyledon immunology
Cysteine Endopeptidases chemistry
Cysteine Endopeptidases metabolism
Models, Structural
Molecular Sequence Data
Organ Specificity
Plant Proteins chemistry
Plant Proteins genetics
Plant Proteins metabolism
Seedlings enzymology
Seedlings genetics
Seedlings growth & development
Seedlings immunology
Sequence Alignment
Agaricales physiology
Cacao enzymology
Cysteine Endopeptidases genetics
Genome, Plant genetics
Plant Diseases immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2690
- Volume :
- 98
- Database :
- MEDLINE
- Journal :
- Plant physiology and biochemistry : PPB
- Publication Type :
- Academic Journal
- Accession number :
- 26691061
- Full Text :
- https://doi.org/10.1016/j.plaphy.2015.11.010