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Naked-eye quantitative aptamer-based assay on paper device.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2016 Apr 15; Vol. 78, pp. 538-546. - Publication Year :
- 2016
-
Abstract
- This work initially describes the design of low-cost, naked-eye quantitative aptamer-based assays by using microfluidic paper-based analytical device (μPAD). Two new detection motifs are proposed for quantitative μPAD measurement without using external electronic readers, which depend on the length of colored region in a strip-like μPAD and the number of colorless detection microzones in a multi-zone μPAD. The length measuring method is based on selective color change of paper from colorless to blue-black via formation of iodine-starch complex. The counting method is conducted on the basis of oxidation-reduction reaction between hydrogen peroxide and potassium permanganate. Their utility is well demonstrated with sensitive, specific detection of adenosine as a model analyte with the naked eye in buffer samples and undiluted human serum. These equipment-free quantitative methods proposed thus hold great potential for the development of more aptamer-based assays that are simple, cost-efficient, portable, and user-friendly for various point-of-care applications particularly in resource-constrained environments.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-4235
- Volume :
- 78
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 26684676
- Full Text :
- https://doi.org/10.1016/j.bios.2015.12.003