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Does the presence of vesicoureteral reflux affect in vitro uropathogenic E. coli growth rate in urine?
- Source :
-
International urology and nephrology [Int Urol Nephrol] 2016 Feb; Vol. 48 (2), pp. 151-4. Date of Electronic Publication: 2015 Dec 17. - Publication Year :
- 2016
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Abstract
- Background: Uroepithelial molecules like uroplakins are involved both in the development of urinary tract and in colonization, attachment and invasion of uropathogenic Escherichia coli (UPEC). Uroplakin disorders are also associated with vesicoureteral reflux (VUR). We hypothesized that urine contents, as well as urinary flow, may be altered in VUR, and aimed to determine whether in vitro UPEC growth is increased in urine from the refluxing systems.<br />Methods: Children evaluated by voiding cystourethrography for UTI were enrolled. Groups 1 and 2 included children with and without VUR, respectively. Sterile urine samples were obtained from all patients, and 2 × 10(2) cfu/mL UPEC suspension was inoculated into these samples. After incubation for 24 h, colony counts were assessed. Both groups were compared for UPEC growth and colony counts.<br />Results: Forty-two urine samples were included (21 in each group). UPEC was cultured in 9 (43 %) and 3 (14 %) samples in Groups 1 and 2, respectively (p = 0.040, OR 4.5). Colony counts were similar in both groups (log x; 2.36 ± 0.25 vs. 2.37 ± 0.12, p = 0.923).<br />Conclusion: Inoculation of 2 × 10(2) cfu UPEC resulted in growth in almost half of the urine samples from refluxing systems, while UPEC growth was inhibited in most urine samples from non-refluxing systems suggesting that urine contents in refluxing units change in such a way that UPEC growth is facilitated.
- Subjects :
- Child, Preschool
Escherichia coli Infections etiology
Escherichia coli Infections urine
Female
Follow-Up Studies
Humans
Infant
Male
Recurrence
Urinalysis
Urinary Tract Infections etiology
Urinary Tract Infections urine
Urothelium microbiology
Urothelium pathology
Vesico-Ureteral Reflux pathology
Escherichia coli Infections microbiology
Urinary Tract Infections microbiology
Uropathogenic Escherichia coli isolation & purification
Vesico-Ureteral Reflux complications
Subjects
Details
- Language :
- English
- ISSN :
- 1573-2584
- Volume :
- 48
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International urology and nephrology
- Publication Type :
- Academic Journal
- Accession number :
- 26681193
- Full Text :
- https://doi.org/10.1007/s11255-015-1168-9