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Rat skeletal muscle lysosomes contain glycogen.
- Source :
-
The International journal of biochemistry [Int J Biochem] 1989; Vol. 21 (5), pp. 561-7. - Publication Year :
- 1989
-
Abstract
- 1. A lysosome- and mitochondria-enriched fraction was obtained from rat skeletal muscle using a differential centrifugation procedure. This fraction contained a proportion (3-4%) of the tissue glycogen content. 2. Lysosomes and mitochondria were separated from one another by centrifugation of a cell-free extract upon discontinuous Ficoll-sucrose gradients. Little glycogen was associated with the resulting mitochondrial fraction. The lysosomal fraction, however, contained a significant amount of glycogen, accounting for 5% of the skeletal muscle glycogen. 3. The lysosomal glycogen was purified and found to be enriched in high molecular weight material. 4. The compartmentation of skeletal muscle glycogen metabolism is suggested.
- Subjects :
- Acid Phosphatase metabolism
Animals
Centrifugation
Centrifugation, Density Gradient
Chemical Phenomena
Chemistry
Glucuronidase metabolism
Mitochondria analysis
Molecular Weight
Proteins metabolism
Rats
alpha-Glucosidases metabolism
Glycogen isolation & purification
Lysosomes analysis
Muscles analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0020-711X
- Volume :
- 21
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The International journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 2668063
- Full Text :
- https://doi.org/10.1016/0020-711x(89)90138-9