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Curcumin upregulates Nrf2 nuclear translocation and protects rat hepatic stellate cells against oxidative stress.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2016 Feb; Vol. 13 (2), pp. 1717-24. Date of Electronic Publication: 2015 Dec 17. - Publication Year :
- 2016
-
Abstract
- The present study aimed to investigate the protective role of curcumin against oxidative stress in rat hepatic stellate cells (HSCs)-T6, and to determine the possible underlying mechanisms. HSC-T6 cells were divided into three groups: Negative control group, oxidant-treated group and curcumin-treated group. Flow cytometry and spectrophotometry were used to measure the production of reactive oxygen species (ROS), and the levels of malondialdehyde (MDA) and glutathione (GSH). Immunocytochemistry and a radioimmunoassay were used to determine the expression of smooth muscle α-actin (α-SMA) and the secretion of extracellular matrix (ECM) molecules. In addition, western blotting and immunocytochemistry were used to determine the expression levels of nuclear factor-erythroid 2-related factor (Nrf2). Treatment with glucose oxidase (GO) significantly stimulated the formation of ROS and increased the production of MDA, as compared with the control cells; however, the production of GSH was only slightly increased. In addition, treatment with GO significantly promoted the expression of α-SMA and the secretion of ECM molecules. Conversely, treatment with curcumin significantly decreased the levels of ROS and MDA, and significantly increased the levels of GSH. Curcumin significantly inhibited the expression of α-SMA and decreased the secretion of ECM molecules. Furthermore, treatment with curcumin significantly increased the nuclear expression levels of Nrf2. These results indicated that curcumin may protect rat HSCs against oxidative stress and inhibit the GO-induced activation and secretion of ECM molecules in vitro. These effects were mediated by the upregulation of Nrf2 nuclear translocation.
- Subjects :
- Actins metabolism
Animals
Cell Line
Cell Nucleus drug effects
Desmin metabolism
Extracellular Matrix drug effects
Extracellular Matrix metabolism
Glucose Oxidase
Hepatic Stellate Cells drug effects
Hepatic Stellate Cells metabolism
Protective Agents pharmacology
Protein Transport drug effects
Rats
Reactive Oxygen Species metabolism
Cell Nucleus metabolism
Curcumin pharmacology
Cytoprotection drug effects
Hepatic Stellate Cells pathology
NF-E2-Related Factor 2 metabolism
Oxidative Stress drug effects
Up-Regulation drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 13
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 26676408
- Full Text :
- https://doi.org/10.3892/mmr.2015.4690