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Inhibition of transcription and translation of globin messenger RNA in dimethyl sulfoxide-stimulated Friend erythroleukemic cells treated with interferon.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1977 May; Vol. 74 (5), pp. 2036-40. - Publication Year :
- 1977
-
Abstract
- The addition of appropriate doses of interferon (IF) to cultures of Friend erythroleukemic cells inhibits dimethyl sulfoxide (Me2SO)-stimulated erythroid differentiation. In this study, the synthesis of heme, hemoglobin, and globin mRNA in Me2SO-stimulated cultures, with or without IF added, was compared. Although the hemoglobin content in Me2SO+IF-treated cultures was reduced 6- to 9-fold compared to that of cultures treated with Me2SO alone, there was less than a 2-fold decrease in the amount of heme accumulated. Globin mRNA, although unchanged in size or base sequence, was reduced in content in the Me2SO+IF cultures. The level of reduction of globin mRNA was insufficient to account for the lack of globin synthesis. Thus, it appears that IF may operate on two levels--one involving the transcription of globin mRNA and the other involving its translation.
- Subjects :
- Animals
Cell Line
Cell Nucleus metabolism
Cytoplasm metabolism
Dimethyl Sulfoxide pharmacology
Friend murine leukemia virus
Globins biosynthesis
Heme biosynthesis
Hemoglobins biosynthesis
Leukemia, Experimental metabolism
Neoplasm Proteins biosynthesis
Interferons pharmacology
Leukemia, Erythroblastic, Acute metabolism
Protein Biosynthesis drug effects
RNA, Messenger metabolism
Transcription, Genetic drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 74
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 266723
- Full Text :
- https://doi.org/10.1073/pnas.74.5.2036