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Effect of Adipocyte Secretome in Melanoma Progression and Vasculogenic Mimicry.
- Source :
-
Journal of cellular biochemistry [J Cell Biochem] 2016 Jul; Vol. 117 (7), pp. 1697-706. Date of Electronic Publication: 2016 Jan 15. - Publication Year :
- 2016
-
Abstract
- Obesity, favored by the modern lifestyle, acquired epidemic proportions nowadays. Obesity has been associated with various major causes of death and morbidity including malignant neoplasms. This increased prevalence has been accompanied by a worldwide increase in cutaneous melanoma incidence rates during the last decades. Obesity involvement in melanoma aetiology has been recognized, but the implicated mechanisms remain unclear. In the present study, we address this relationship and investigate the influence of adipocytes secretome on B16-F10 and MeWo melanoma cell lines. Using the 3T3-L1 adipocyte cell line, as well as ex vivo subcutaneous (SAT) and visceral (VAT) adipose tissue conditioned medium, we were able to show that adipocyte-released factors play a dual role in increasing melanoma cell overall survival, both by enhancing proliferation and decreasing apoptosis. B16-F10 cell migration and cell-cell and cell-matrix adhesion capacity were predominantly enhanced in the presence of SAT and VAT released factors. Melanocytes morphology and melanin content were also altered by exposure to adipocyte conditioned medium disclosing a more dedifferentiated phenotype of melanocytes. In addition, exposure to adipocyte-secreted molecules induced melanocytes to rearrange, on 3D cultures, into vessel-like structures, and generate characteristic vasculogenic mimicry patterns. These findings are corroborated by the released factors profile of 3T3-L1, SAT, and VAT assessed by microarrays, and led us to highlight the mechanisms by which adipose secretome from sub-cutaneous or visceral depots promote melanoma progression. J. Cell. Biochem. 117: 1697-1706, 2016. © 2015 Wiley Periodicals, Inc.<br /> (© 2015 Wiley Periodicals, Inc.)
- Subjects :
- 3T3-L1 Cells
Adipocytes pathology
Animals
Cell Dedifferentiation
Cell Line, Tumor
Intra-Abdominal Fat pathology
Melanocytes metabolism
Melanocytes pathology
Melanoma genetics
Melanoma pathology
Mice
Neovascularization, Pathologic genetics
Neovascularization, Pathologic pathology
Obesity complications
Obesity genetics
Obesity pathology
Subcutaneous Fat pathology
Adipocytes metabolism
Intra-Abdominal Fat metabolism
Melanoma metabolism
Neovascularization, Pathologic metabolism
Obesity metabolism
Subcutaneous Fat metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4644
- Volume :
- 117
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of cellular biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 26666522
- Full Text :
- https://doi.org/10.1002/jcb.25463