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Effects of renin inhibitors on the expression of kidney renin gene and tissue renin-like activity.
- Source :
-
Journal of hypertension. Supplement : official journal of the International Society of Hypertension [J Hypertens Suppl] 1989 Apr; Vol. 7 (2), pp. S29-32. - Publication Year :
- 1989
-
Abstract
- The effect of renin inhibitor ES-1005 on renin gene expression was investigated in sodium-depleted marmosets. The kidneys were removed after continuous infusion of ES-1005 (12 mg/kg per h) for 2 h. The relative amount of kidney renin messenger (m)RNA was measured by densitometric Northern blot analysis using an alpha-32P-labelled human renin complementary (c)DNA fragment as a hybridization probe. Plasma renin activity was completely inhibited by ES-1005. The level of kidney renin mRNA decreased significantly to about one-third of the normal control value. We also investigated the inhibitory potency of the renin inhibitor ES-6864 on the renin-like activity in dog tissues (adrenal glands, aorta and brainstem). ES-6864 inhibited the tissue renin-like activity with an IC50 of 10(-7) to 10(-8) mol/l in vitro. Renin inhibitors not only inhibit the activity of plasma and tissue renin, but also suppress the synthesis of renin in the kidney.
- Subjects :
- Animals
Blood Pressure drug effects
Blotting, Northern
Callithrix
Captopril pharmacology
Dogs
Female
Kidney ultrastructure
Male
RNA, Messenger biosynthesis
Renin genetics
Renin metabolism
Sodium deficiency
Kidney drug effects
Oligopeptides pharmacology
RNA, Messenger drug effects
Renin antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 0952-1178
- Volume :
- 7
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of hypertension. Supplement : official journal of the International Society of Hypertension
- Publication Type :
- Academic Journal
- Accession number :
- 2666610
- Full Text :
- https://doi.org/10.1097/00004872-198904002-00007