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The tumor suppressor BTG1 is expressed in the developing digits and regulates skeletogenic differentiation of limb mesodermal progenitors in high density cultures.
- Source :
-
Cell and tissue research [Cell Tissue Res] 2016 May; Vol. 364 (2), pp. 299-308. Date of Electronic Publication: 2015 Dec 11. - Publication Year :
- 2016
-
Abstract
- In the developing limb, differentiation of skeletal progenitors towards distinct connective tissues of the digits is correlated with the establishment of well-defined domains of Btg1 gene expression. Zones of high expression of Btg1 include the earliest digit blastemas, the condensing mesoderm at the tip of the growing digits, the peritendinous mesenchyme, and the chondrocytes around the developing interphalangeal joints. Gain- and loss-of function experiments in micromass cultures of skeletal progenitors reveal a negative influence of Btg1 in cartilage differentiation accompanied by up-regulation of Ccn1, Scleraxis and PTHrP. Previous studies have assigned a role to these factors in the aggregation of progenitors in the digit tips (Ccn1), in the differentiation of tendon blastemas (Scleraxis) and repressing hypertrophic cartilage differentiation (PTHrP). Overexpression of Btg1 up-regulates the expression of retinoic acid and thyroid hormone receptors, but, different from other systems, the influence of BTG1 in connective tissue differentiation appears to be independent of retinoic acid and thyroid hormone signaling.
- Subjects :
- Animals
Basic Helix-Loop-Helix Transcription Factors biosynthesis
Cell Differentiation physiology
Cell Proliferation physiology
Cells, Cultured
Chick Embryo
Chondrocytes cytology
Chondrocytes metabolism
Cysteine-Rich Protein 61 biosynthesis
Gene Expression Regulation, Developmental
Mice
Mice, Inbred C57BL
Neoplasm Proteins biosynthesis
Parathyroid Hormone-Related Protein biosynthesis
Receptors, Thyroid Hormone biosynthesis
Signal Transduction physiology
Tretinoin metabolism
Cartilage cytology
Chondrogenesis physiology
Extremities embryology
Mesoderm metabolism
Neoplasm Proteins metabolism
Toes embryology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0878
- Volume :
- 364
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cell and tissue research
- Publication Type :
- Academic Journal
- Accession number :
- 26662056
- Full Text :
- https://doi.org/10.1007/s00441-015-2331-4