Enhanced peroxidase-like activity of porphyrin functionalized ceria nanorods for sensitive and selective colorimetric detection of glucose.

Ceria nanorods modified with 5,10,15,20-tetrakis(4-carboxyl phenyl)-porphyrin (H2TCPP) were prepared. These nanocomposites (H2TCPP-CeO2) exhibited the intrinsic peroxidase-like activity and could catalyze the oxidation of classical peroxidase substrate 3,3',5,5'-tetramethylbiphenyl dihydrochloride (TMB·2HCl) in the presence of H2O2 to produce a typical color reaction from colorless to blue. Our results demonstrated that both the H2TCPP-CeO2 nanocomposites and CeO2 nanorods exhibited higher thermal durance than that of HRP. The affinity of The H2TCPP-CeO2 nanocomposites toward H2O2 and TMB is similar to that of HRP. Fluorescent results indicated that the catalytic mechanism of the H2TCPP-CeO2 nanocomposites were from the decomposition of H2O2 into hydroxyl radicals. Based on these studies, a simple, sensitive, and selective visual and colorimetric method using TMB as the substrate was designed to detect glucose when combined with glucose oxidase. The proposed colorimetric method can detect H2O2 at a low detection limit of 6.1×10(-6)M and a dynamic range of 10(-5)-10(-4)mol·L(-1). This method can also detect glucose at a low detection limit of 3.3×10(-5)mol·L(-1) and a dynamic range of 5.0×10(-5)-1.0×10(-4)mol·L(-1).
(Copyright © 2015 Elsevier B.V. All rights reserved.)