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Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from Anaerobic Granular Sludge.
- Source :
-
Microbes and environments [Microbes Environ] 2015; Vol. 30 (4), pp. 335-8. Date of Electronic Publication: 2015 Dec 04. - Publication Year :
- 2015
-
Abstract
- The aim of the present study was to investigate the prokaryotic community structure of the anaerobic ciliate, Metopus sp. using rRNA sequencing, fluorescence in situ hybridization (FISH), and transmission electron microscopy (TEM). Metopus sp. was physically separated from anaerobic granular sludge in a domestic wastewater treatment plant and anoxically cultivated for 7 d. 16S rRNA gene sequences from the prokaryotes Methanoregula boonei and Clostridium aminobutyricum were abundantly detected in Metopus ciliates. The FISH analysis using the oligonucleotide probes Mg1200b and Cla568 demonstrated that these prokaryotes were localized within Metopus cells. These results identify M. boonei- and C. aminobutyricum-like prokaryotes as novel endosymbionts of Metopus ciliates.
- Subjects :
- Clostridium classification
Clostridium genetics
Cluster Analysis
DNA, Archaeal chemistry
DNA, Archaeal genetics
DNA, Bacterial chemistry
DNA, Bacterial genetics
DNA, Ribosomal chemistry
DNA, Ribosomal genetics
In Situ Hybridization, Fluorescence
Methanomicrobiales classification
Methanomicrobiales genetics
Microbial Consortia
Microscopy, Electron, Transmission
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Biota
Ciliophora microbiology
Clostridium isolation & purification
Methanomicrobiales isolation & purification
Sewage microbiology
Symbiosis
Subjects
Details
- Language :
- English
- ISSN :
- 1347-4405
- Volume :
- 30
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Microbes and environments
- Publication Type :
- Academic Journal
- Accession number :
- 26639580
- Full Text :
- https://doi.org/10.1264/jsme2.ME15154