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Effect of (+)-dehydrofukinone on GABAA receptors and stress response in fish model.
- Source :
-
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas [Braz J Med Biol Res] 2016 Jan; Vol. 49 (1), pp. e4872. Date of Electronic Publication: 2015 Nov 27. - Publication Year :
- 2016
-
Abstract
- (+)-Dehydrofukinone (DHF) is a major component of the essential oil of Nectandra grandiflora (Lauraceae), and exerts a depressant effect on the central nervous system of fish. However, the neuronal mechanism underlying DHF action remains unknown. This study aimed to investigate the action of DHF on GABAA receptors using a silver catfish (Rhamdia quelen) model. Additionally, we investigated the effect of DHF exposure on stress-induced cortisol modulation. Chemical identification was performed using gas chromatography-mass spectrometry and purity was evaluated using gas chromatography with a flame ionization detector. To an aquarium, we applied between 2.5 and 50 mg/L DHF diluted in ethanol, in combination with 42.7 mg/L diazepam. DHF within the range of 10-20 mg/L acted collaboratively in combination with diazepam, but the sedative action of DHF was reversed by 3 mg/L flumazenil. Additionally, fish exposed for 24 h to 2.5-20 mg/L DHF showed no side effects and there was sustained sedation during the first 12 h of drug exposure with 10-20 mg/L DHF. DHF pretreatment did not increase plasma cortisol levels in fish subjected to a stress protocol. Moreover, the stress-induced cortisol peak was absent following pretreatment with 20 mg/L DHF. DHF proved to be a relatively safe sedative or anesthetic, which interacts with GABAergic and cortisol pathways in fish.
- Subjects :
- Anesthetics pharmacology
Animals
Diazepam pharmacology
Flame Ionization
Flumazenil pharmacology
GABA Modulators pharmacology
Gas Chromatography-Mass Spectrometry
Hydrocortisone blood
Hypnotics and Sedatives pharmacology
Oils, Volatile isolation & purification
Plant Extracts chemistry
Plant Leaves chemistry
Catfishes metabolism
Hydrocortisone metabolism
Lauraceae chemistry
Oils, Volatile administration & dosage
Receptors, GABA-A drug effects
Sesquiterpenes pharmacology
Stress, Physiological drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1414-431X
- Volume :
- 49
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
- Publication Type :
- Academic Journal
- Accession number :
- 26628396
- Full Text :
- https://doi.org/10.1590/1414-431X20154872