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Analysis of mitochondrial structure and function in the Drosophila larval musculature.
- Source :
-
Mitochondrion [Mitochondrion] 2016 Jan; Vol. 26, pp. 33-42. Date of Electronic Publication: 2015 Dec 01. - Publication Year :
- 2016
-
Abstract
- Mitochondria are dynamic organelles that change their architecture in normal physiological conditions. Mutations in genes that control mitochondrial fission or fusion, such as dynamin-related protein (Drp1), Mitofusins 1 (Mfn1) and 2 (Mfn2), and Optic atrophy 1 (Opa1), result in neuropathies or neurodegenerative diseases. It is increasingly clear that altered mitochondrial dynamics also underlie the pathology of other degenerative diseases, including Parkinson's disease (PD). Thus, understanding mitochondrial distribution, shape, and dynamics in all cell types is a prerequisite for developing and defining treatment regimens that may differentially affect tissues. The majority of Drosophila genes implicated in mitochondrial dynamics have been studied in the adult indirect flight muscle (IFM). Here, we discuss the utility of Drosophila third instar larvae (L3) as an alternative model to analyze and quantify mitochondrial behaviors. Advantages include large muscle cell size, a stereotyped arrangement of mitochondria that is conserved in mammalian muscles, and the ability to analyze muscle-specific gene function in mutants that are lethal prior to adult stages. In particular, we highlight methods for sample preparation and analysis of mitochondrial morphological features.<br /> (Copyright © 2015 Elsevier B.V. and Mitochondria Research Society. All rights reserved.)
- Subjects :
- Animals
Drosophila melanogaster
Larva genetics
Larva metabolism
Larva ultrastructure
Drosophila Proteins genetics
Drosophila Proteins metabolism
Mitochondria, Muscle genetics
Mitochondria, Muscle metabolism
Mitochondria, Muscle ultrastructure
Mitochondrial Proteins genetics
Mitochondrial Proteins metabolism
Muscles metabolism
Muscles ultrastructure
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8278
- Volume :
- 26
- Database :
- MEDLINE
- Journal :
- Mitochondrion
- Publication Type :
- Academic Journal
- Accession number :
- 26611999
- Full Text :
- https://doi.org/10.1016/j.mito.2015.11.005