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Synchrotron X-ray microscopy reveals early calcium and iron interaction with crocidolite fibers in the lung of exposed mice.
- Source :
-
Toxicology letters [Toxicol Lett] 2016 Jan 22; Vol. 241, pp. 111-20. Date of Electronic Publication: 2015 Nov 18. - Publication Year :
- 2016
-
Abstract
- Human exposure to asbestos can cause a wide variety of lung diseases that are still a current major health concern, even if asbestos has been banned in many countries. It has been shown in many studies that asbestos fibers, ingested by alveolar macrophages, disrupt lung iron homeostasis by sequestering iron. Calcium can also be deposited on the fibers. The pathways along which iron and above all calcium interact with fibers are still unknown. Our aim was that of investigating if the iron accumulation induced by the inhaled asbestos fibers also involves calcium ions accumulation. Lung sections of asbestos-exposed mice were analyzed using an extremely sensitive procedure available at the synchrotron facilities, that provides morphological and chemical information based on X-ray fluorescence microspectroscopy (μ-XRF). In this study we show that (1) where conventional histochemical procedures revealed only weak deposits of iron and calcium, μ-XRF analysis is able to detect significant deposits of both iron and calcium on the inhaled asbestos fibers; (2) the extent of the deposition of these ions is proportionally directly related and (3) iron and calcium deposition on inhaled asbestos fibers is concomitant with the appearance of inflammatory and hyperplastic reactions.<br /> (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Animals
Calcium metabolism
Homeostasis drug effects
Humans
Inhalation Exposure
Iron metabolism
Macrophages, Alveolar drug effects
Macrophages, Alveolar ultrastructure
Male
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence
Tissue Distribution
X-Rays
Zinc metabolism
Asbestos, Crocidolite toxicity
Asbestosis pathology
Calcium chemistry
Iron chemistry
Lung pathology
Lung Diseases chemically induced
Lung Diseases pathology
Microscopy instrumentation
Synchrotrons instrumentation
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3169
- Volume :
- 241
- Database :
- MEDLINE
- Journal :
- Toxicology letters
- Publication Type :
- Academic Journal
- Accession number :
- 26602167
- Full Text :
- https://doi.org/10.1016/j.toxlet.2015.11.016