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Design and performance of an ultra-flexible two-photon microscope for in vivo research.

Design and performance of an ultra-flexible two-photon microscope for in vivo research.

Authors :
Mayrhofer JM
Haiss F
Haenni D
Weber S
Zuend M
Barrett MJ
Ferrari KD
Maechler P
Saab AS
Stobart JL
Wyss MT
Johannssen H
Osswald H
Palmer LM
Revol V
Schuh CD
Urban C
Hall A
Larkum ME
Rutz-Innerhofer E
Zeilhofer HU
Ziegler U
Weber B
Source :
Biomedical optics express [Biomed Opt Express] 2015 Oct 02; Vol. 6 (11), pp. 4228-37. Date of Electronic Publication: 2015 Oct 02 (Print Publication: 2015).
Publication Year :
2015

Abstract

We present a cost-effective in vivo two-photon microscope with a highly flexible frontend for in vivo research. Our design ensures fast and reproducible access to the area of interest, including rotation of imaging plane, and maximizes space for auxiliary experimental equipment in the vicinity of the animal. Mechanical flexibility is achieved with large motorized linear stages that move the objective in the X, Y, and Z directions up to 130 mm. 360° rotation of the frontend (rotational freedom for one axis) is achieved with the combination of a motorized high precision bearing and gearing. Additionally, the modular design of the frontend, based on commercially available optomechanical parts, allows straightforward updates to future scanning technologies. The design exceeds the mobility of previous movable microscope designs while maintaining high optical performance.

Details

Language :
English
ISSN :
2156-7085
Volume :
6
Issue :
11
Database :
MEDLINE
Journal :
Biomedical optics express
Publication Type :
Academic Journal
Accession number :
26600989
Full Text :
https://doi.org/10.1364/BOE.6.004228