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HIV-1 Tat regulates the expression of the dcw operon and stimulates the proliferation of bacteria.
- Source :
-
Microbial pathogenesis [Microb Pathog] 2016 Jan; Vol. 90, pp. 34-40. Date of Electronic Publication: 2015 Nov 17. - Publication Year :
- 2016
-
Abstract
- Infections of pathogenic bacteria are very common in acquired immunodeficiency syndrome (AIDS) patients. However, the biological effects of HIV-1 Tat on bacteria are incompletely understood. In this study, HIV-1 Tat was expressed in Escherichia coli and Pseudomonas aeruginosa (PA01) to investigate its biological effects on bacteria. Bacterial cells expressing either HIV-1 Tat1-86 (Tat1-86) or HIV-1 Tat1-72 (Tat1-72) grow significantly faster than those with either only an empty vector or an unrelated control (GFP or Rluc). Supplementation of purified HIV-1 Tat1-86 or Tat1-101 protein into bacterial culture medium stimulated the growth of both E. coli and PA01. The expression profile of certain cell division-associated genes, such as those in the division cell wall (dcw) operon (ftsA, ftsQ, ftsW and ftsZ), yafO and zipA, was altered in HIV-1 Tat1-86 expressing E. coli BL21(DE3). Furthermore, the expression of firefly luciferase (Fluc) reporter gene, when engineered for control by the dcw promoter and terminator, was enhanced by HIV-1 Tat in E. coli, confirming that HIV-1 Tat transcriptionally regulates the expression of the dcw operon. The finding that HIV-1 Tat stimulates bacterial growth whether it is produced intracellularly or applied extracellularly may have relevance for HIV patients who are highly susceptible to opportunistic bacterial infections. Contents category: Viruses -Retroviruses. The GenBank accession number for the sequence of HIV-1 Tat1-86 is AF324439.1.<br /> (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Subjects :
- Bacterial Proteins biosynthesis
Bacterial Proteins genetics
Cell Division drug effects
Cell Division physiology
Cell Wall metabolism
Endogenous Retroviruses genetics
Escherichia coli genetics
Escherichia coli metabolism
HIV-1 genetics
Peptide Fragments pharmacology
Pseudomonas aeruginosa genetics
Pseudomonas aeruginosa metabolism
Transcription, Genetic
Transcriptional Activation
tat Gene Products, Human Immunodeficiency Virus biosynthesis
tat Gene Products, Human Immunodeficiency Virus genetics
tat Gene Products, Human Immunodeficiency Virus pharmacology
Cell Wall genetics
Escherichia coli cytology
HIV-1 physiology
Operon
Pseudomonas aeruginosa cytology
tat Gene Products, Human Immunodeficiency Virus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1208
- Volume :
- 90
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 26596708
- Full Text :
- https://doi.org/10.1016/j.micpath.2015.11.005