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Inmirania thermothiophila gen. nov., sp. nov., a thermophilic, facultatively autotrophic, sulfur-oxidizing gammaproteobacterium isolated from a shallow-sea hydrothermal vent.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2016 Feb; Vol. 66 (2), pp. 701-706. Date of Electronic Publication: 2015 Nov 17. - Publication Year :
- 2016
-
Abstract
- A novel thermophilic, facultatively autotrophic bacterium, strain S2479T, was isolated from a thermal spring located in a tidal zone of a geothermally heated beach (Kuril Islands, Russia). Cells of strain S2479T were rod-shaped and motile with a Gram-negative cell-wall type. The temperature range for growth was 35-68 °C (optimum 65 °C), and the pH range for growth was pH 5.5-8.8 (optimum pH 6.5). Growth of strain S2479T was observed in the presence of NaCl concentrations ranging from 0.5 to 3.5 % (w/v) (optimum 1.5-2.0 %). The strain oxidized sulfur and thiosulfate as sole energy sources for autotrophic growth under anaerobic conditions with nitrate as electron acceptor. Strain S2479T was also capable of heterotrophic growth by reduction of nitrate with oxidation of low-chain fatty acids and a limited number of other carboxylic acids or with complex proteinaceous compounds. Nitrate was reduced to N2. Sulfur compounds were oxidized to sulfate. Strain S2479T did not grow aerobically during incubation at atmospheric concentration of oxygen but was able to grow microaerobically (1 % of oxygen in gas phase). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain was a member of the family Ectothiorhodospiraceae, order Chromatiales, class Gammaproteobacteria. On the basis of phylogenetic and phenotypic properties, strain S2479T represents a novel species of a new genus, for which the name Inmirania thermothiophila gen. nov., sp. nov. is proposed. The type strain of the type species is S2479T ( = DSM 100275T = VKM B-2962T).
- Subjects :
- Autotrophic Processes
Bacterial Typing Techniques
Base Composition
DNA, Bacterial genetics
Fatty Acids chemistry
Gammaproteobacteria genetics
Gammaproteobacteria isolation & purification
Oxidation-Reduction
RNA, Ribosomal, 16S genetics
Russia
Sequence Analysis, DNA
Sulfur Compounds metabolism
Sulfur-Reducing Bacteria classification
Sulfur-Reducing Bacteria genetics
Sulfur-Reducing Bacteria isolation & purification
Gammaproteobacteria classification
Hydrothermal Vents microbiology
Phylogeny
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 66
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 26582356
- Full Text :
- https://doi.org/10.1099/ijsem.0.000773