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High-resolution copy number analysis of paired normal-tumor samples from diffuse large B cell lymphoma.
- Source :
-
Annals of hematology [Ann Hematol] 2016 Jan; Vol. 95 (2), pp. 253-62. Date of Electronic Publication: 2015 Nov 14. - Publication Year :
- 2016
-
Abstract
- Copy number analysis can be useful for assessing prognosis in diffuse large B cell lymphoma (DLBCL). We analyzed copy number data from tumor samples of 60 patients diagnosed with DLBCL de novo and their matched normal samples. We detected 63 recurrent copy number alterations (CNAs), including 33 gains, 30 losses, and nine recurrent acquired copy number neutral loss of heterozygosity (CNN-LOH). Interestingly, 20 % of cases acquired CNN-LOH of 6p21 locus, which involves the HLA region. In normal cells, there were no CNAs but we observed CNN-LOH involving some key lymphoma regions such as 6p21 and 9p24.1 (5 %) and 17p13.1 (2.5 %) in DLBCL patients. Furthermore, a model with some specific CNA was able to predict the subtype of DLBCL, 1p36.32 and 10q23.31 losses being restricted to germinal center B cell-like (GCB) DLBCL. In contrast, 8p23.3 losses and 11q24.3 gains were strongly associated with the non-GCB subtype. A poor prognosis was associated with biallelic inactivation of TP53 or 18p11.32 losses, while prognosis was better in cases carrying 11q24.3 gains. In summary, CNA abnormalities identify specific DLBCL groups, and we describe CNN-LOH in germline cells from DLBCL patients that are associated with genes that probably play a key role in DLBCL development.
- Subjects :
- Adult
Aged
Aged, 80 and over
Comparative Genomic Hybridization methods
Female
Follow-Up Studies
Gene Expression Profiling methods
Humans
Male
Middle Aged
Oligonucleotide Array Sequence Analysis methods
DNA Copy Number Variations genetics
Lymphoma, Large B-Cell, Diffuse diagnosis
Lymphoma, Large B-Cell, Diffuse genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0584
- Volume :
- 95
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Annals of hematology
- Publication Type :
- Academic Journal
- Accession number :
- 26573278
- Full Text :
- https://doi.org/10.1007/s00277-015-2552-3