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Homing of allogeneic nestin-positive hair follicle-associated pluripotent stem cells after maternal transplantation in experimental model of cortical dysplasia.
- Source :
-
Biochemistry and cell biology = Biochimie et biologie cellulaire [Biochem Cell Biol] 2015 Dec; Vol. 93 (6), pp. 619-25. Date of Electronic Publication: 2015 Oct 13. - Publication Year :
- 2015
-
Abstract
- An embryo has the capability to accept allo- or xeno-geneic cells, which probably makes it an ideal candidate for stem cell transplantation of various cerebral cortex abnormalities, such as cortical dysplasia. The aim of this study was to determine hair follicle-associated pluripotent (HAP) stem cells homing into various organs of mother and fetus. Cells were obtained, analyzed for immunophenotypic features, and then labelled with CM-Dil; nestin(+)HAP stem cells or media phosphate-buffered saline (PBS) were intravenously delivered on day 16 of gestation in BALB/c mice, which intraperitoneally received methylazoxymethanol (MAM) one day in advance, and homing was assessed at 24 h after cell injection. Flow cytometry and immunocytochemistry manifested positive expression of nestin in HAP stem cells. For both mother and fetus, brain, lungs, liver, and spleen were the host organs for cell implants. For the brain, the figure was considerably higher in fetus, 4.05 ± 0.5% (p ≤ 0.05 vs. mother). MAM-injected mice had a downward trend for SDF-1α and CXCR4 (p ≤ 0.05 vs. control), but HAP stem cells group showed an upward trend for CXCR4 (p ≤ 0.05 vs. MAM). We conclude the HAP stem cells show homing potential in experimental cortical dysplasia, which may permit these cells to be a target in future work on prenatal therapy of neural disorders.
- Subjects :
- Animals
Brain embryology
Brain immunology
Brain metabolism
Brain pathology
Carbocyanines chemistry
Female
Flow Cytometry
Fluorescent Dyes chemistry
Immunohistochemistry
Malformations of Cortical Development embryology
Malformations of Cortical Development immunology
Malformations of Cortical Development pathology
Mice, Inbred BALB C
Microscopy, Fluorescence
Neurons immunology
Neurons metabolism
Neurons pathology
Pluripotent Stem Cells cytology
Pluripotent Stem Cells immunology
Pluripotent Stem Cells metabolism
Pregnancy
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Disease Models, Animal
Fetal Therapies
Hair Follicle cytology
Malformations of Cortical Development therapy
Maternal-Fetal Exchange
Nestin metabolism
Pluripotent Stem Cells transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 1208-6002
- Volume :
- 93
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biochemistry and cell biology = Biochimie et biologie cellulaire
- Publication Type :
- Academic Journal
- Accession number :
- 26568364
- Full Text :
- https://doi.org/10.1139/bcb-2015-0098