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An in vitro screening with emerging contaminants reveals inhibition of carboxylesterase activity in aquatic organisms.
- Source :
-
Aquatic toxicology (Amsterdam, Netherlands) [Aquat Toxicol] 2015 Dec; Vol. 169, pp. 215-22. Date of Electronic Publication: 2015 Nov 04. - Publication Year :
- 2015
-
Abstract
- Pharmaceuticals and personal care products (PPCPs) form part of the new generation of pollutants present in many freshwater and marine ecosystems. Although environmental concentrations of these bioactive substances are low, they cause sublethal effects (e.g., enzyme inhibition) in non-target organisms. However, little is known on metabolism of PPCPs by non-mammal species. Herein, an in vitro enzyme trial was performed to explore sensitivity of carboxylesterase (CE) activity of aquatic organisms to fourteen PPCPs. The esterase activity was determined in the liver of Mediterranean freshwater fish (Barbus meridionalis and Squalius laietanus), coastal marine fish (Dicentrarchus labrax and Solea solea), middle-slope fish (Trachyrhynchus scabrus), deep-sea fish (Alepocephalus rostratus and Cataetix laticeps), and in the digestive gland of a decapod crustacean (Aristeus antennatus). Results showed that 100μM of the lipid regulators simvastatin and fenofibrate significantly inhibited (30-80% of controls) the CE activity of all target species. Among the personal care products, nonylphenol and triclosan were strong esterase inhibitors in most species (36-68% of controls). Comparison with literature data suggests that fish CE activity is as sensitive to inhibition by some PPCPs as that of mammals, although their basal activity levels are lower than in mammals. Pending further studies on the interaction between PPCPs and CE activity, we postulate that this enzyme may act as a molecular sink for certain PPCPs in a comparable way than that described for the organophosphorus pesticides.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Aquatic Organisms enzymology
Carboxylic Ester Hydrolases antagonists & inhibitors
Cyprinidae physiology
Decapoda drug effects
Enzyme Activation drug effects
Flatfishes physiology
Fresh Water chemistry
Liver drug effects
Phenols toxicity
Triclosan toxicity
Aquatic Organisms drug effects
Carboxylic Ester Hydrolases metabolism
Water Pollutants, Chemical toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1514
- Volume :
- 169
- Database :
- MEDLINE
- Journal :
- Aquatic toxicology (Amsterdam, Netherlands)
- Publication Type :
- Academic Journal
- Accession number :
- 26562051
- Full Text :
- https://doi.org/10.1016/j.aquatox.2015.11.001