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Application of Pseudomonas fluorescens to Blackberry under Field Conditions Improves Fruit Quality by Modifying Flavonoid Metabolism.
- Source :
-
PloS one [PLoS One] 2015 Nov 11; Vol. 10 (11), pp. e0142639. Date of Electronic Publication: 2015 Nov 11 (Print Publication: 2015). - Publication Year :
- 2015
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Abstract
- Application of a plant growth promoting rhizobacterium (PGPR), Pseudomonas fluorescens N21.4, to roots of blackberries (Rubus sp.) is part of an optimised cultivation practice to improve yields and quality of fruit throughout the year in this important fruit crop. Blackberries are especially rich in flavonoids and therefore offer potential benefits for human health in prevention or amelioration of chronic diseases. However, the phenylpropanoid pathway and its regulation during ripening have not been studied in detail, in this species. PGPR may trigger flavonoid biosynthesis as part of an induced systemic response (ISR) given the important role of this pathway in plant defence, to cause increased levels of flavonoids in the fruit. We have identified structural genes encoding enzymes of the phenylpropanoid and flavonoid biosynthetic pathways catalysing the conversion of phenylalanine to the final products including flavonols, anthocyanins and catechins from blackberry, and regulatory genes likely involved in controlling the activity of pathway branches. We have also measured the major flavonols, anthocyanins and catechins at three stages during ripening. Our results demonstrate the coordinated expression of flavonoid biosynthetic genes with the accumulation of anthocyanins, catechins, and flavonols in developing fruits of blackberry. Elicitation of blackberry plants by treatment of roots with P.fluorescens N21.4, caused increased expression of some flavonoid biosynthetic genes and an accompanying increase in the concentration of selected flavonoids in fruits. Our data demonstrate the physiological mechanisms involved in the improvement of fruit quality by PGPR under field conditions, and highlight some of the genetic targets of elicitation by beneficial bacteria.
- Subjects :
- Anthocyanins chemistry
Anthocyanins metabolism
Catechin chemistry
Catechin metabolism
Chromatography, High Pressure Liquid
Flavonoids metabolism
Flavonols chemistry
Flavonols metabolism
Fruit chemistry
Fruit metabolism
Mass Spectrometry
Plant Proteins genetics
Plant Proteins metabolism
Plant Roots growth & development
Plant Roots metabolism
Plant Roots microbiology
Pseudomonas fluorescens isolation & purification
Real-Time Polymerase Chain Reaction
Rubus chemistry
Rubus growth & development
Rubus metabolism
Symbiosis physiology
Flavonoids chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 26559418
- Full Text :
- https://doi.org/10.1371/journal.pone.0142639