Quantitative MS analysis of therapeutic mAbs and their glycosylation for pharmacokinetics study.

Authors :: Cong Y
Zhang Z
Zhang S
Hu L
Gu J
Source :: Proteomics. Clinical applications [Proteomics Clin Appl] 2016 Apr; Vol. 10 (4), pp. 303-14. Date of Electronic Publication: 2015 Dec 10.
Publication Year :: 2016
Abstract: Therapeutic mAbs play an important role in the treatment of a wide range of diseases. Due to their complexity, comprehensive evaluation of their pharmacokinetics has yet to be fully achieved. It is crucial to develop sensitive, accurate, reliable, and reproducible methods for quantitation of mAbs in complex samples. In addition, it is also important to evaluate the PTMs which can affect their safety and/or effectiveness. MS-based methods provide an emerging approach for quantitation of proteins and their modification forms. In this review, we give a brief overview of quantification analysis of mAbs in complex biological samples and the characterization of N-glycosylation by biological MS.<br /> (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Subjects :: Antibodies, Monoclonal pharmacokinetics
Antibodies, Monoclonal therapeutic use
Chromatography, Liquid
Glycosylation
Half-Life
Humans
Isotope Labeling
Mass Spectrometry
Proteolysis
Trypsin chemistry
Antibodies, Monoclonal analysis
Immunoglobulin Fab Fragments analysis
Immunoglobulin Fc Fragments analysis
Peptide Fragments analysis
Polysaccharides analysis
Protein Processing, Post-Translational

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