Back to Search
Start Over
Comprehensive Molecular Characterization of Papillary Renal-Cell Carcinoma.
- Source :
-
The New England journal of medicine [N Engl J Med] 2016 Jan 14; Vol. 374 (2), pp. 135-45. Date of Electronic Publication: 2015 Nov 04. - Publication Year :
- 2016
-
Abstract
- Background: Papillary renal-cell carcinoma, which accounts for 15 to 20% of renal-cell carcinomas, is a heterogeneous disease that consists of various types of renal cancer, including tumors with indolent, multifocal presentation and solitary tumors with an aggressive, highly lethal phenotype. Little is known about the genetic basis of sporadic papillary renal-cell carcinoma, and no effective forms of therapy for advanced disease exist.<br />Methods: We performed comprehensive molecular characterization of 161 primary papillary renal-cell carcinomas, using whole-exome sequencing, copy-number analysis, messenger RNA and microRNA sequencing, DNA-methylation analysis, and proteomic analysis.<br />Results: Type 1 and type 2 papillary renal-cell carcinomas were shown to be different types of renal cancer characterized by specific genetic alterations, with type 2 further classified into three individual subgroups on the basis of molecular differences associated with patient survival. Type 1 tumors were associated with MET alterations, whereas type 2 tumors were characterized by CDKN2A silencing, SETD2 mutations, TFE3 fusions, and increased expression of the NRF2-antioxidant response element (ARE) pathway. A CpG island methylator phenotype (CIMP) was observed in a distinct subgroup of type 2 papillary renal-cell carcinomas that was characterized by poor survival and mutation of the gene encoding fumarate hydratase (FH).<br />Conclusions: Type 1 and type 2 papillary renal-cell carcinomas were shown to be clinically and biologically distinct. Alterations in the MET pathway were associated with type 1, and activation of the NRF2-ARE pathway was associated with type 2; CDKN2A loss and CIMP in type 2 conveyed a poor prognosis. Furthermore, type 2 papillary renal-cell carcinoma consisted of at least three subtypes based on molecular and phenotypic features. (Funded by the National Institutes of Health.).
- Subjects :
- Carcinoma, Papillary genetics
CpG Islands physiology
DNA Methylation
Humans
Kidney Neoplasms genetics
MicroRNAs chemistry
NF-E2-Related Factor 2 genetics
Phenotype
Proto-Oncogene Proteins c-met chemistry
Proto-Oncogene Proteins c-met genetics
RNA, Messenger chemistry
RNA, Neoplasm chemistry
Sequence Analysis, RNA
Signal Transduction physiology
Carcinoma, Papillary metabolism
Kidney Neoplasms metabolism
Mutation
NF-E2-Related Factor 2 metabolism
Proto-Oncogene Proteins c-met metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1533-4406
- Volume :
- 374
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The New England journal of medicine
- Publication Type :
- Academic Journal
- Accession number :
- 26536169
- Full Text :
- https://doi.org/10.1056/NEJMoa1505917