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Photodynamic inactivation of gramicidin channels in bilayer lipid membranes: protective efficacy of singlet oxygen quenchers depends on photosensitizer location.
- Source :
-
Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2015 Jun; Vol. 80 (6), pp. 745-51. - Publication Year :
- 2015
-
Abstract
- The impact of double bonds in fatty acyl tails of unsaturated lipids on the photodynamic inactivation of ion channels formed by the pentadecapeptide gramicidin A in a planar bilayer lipid membrane was studied. The presence of unsaturated acyl tails protected gramicidin A against photodynamic inactivation, with efficacy depending on the depth of a photosensitizer in the membrane. The protective effect of double bonds was maximal with membrane-embedded chlorin e6-monoethylenediamine monoamide dimethyl ester, and minimal - in the case of water-soluble tri-sulfonated aluminum phthalocyanine (AlPcS3) known to reside at the membrane surface. By contrast, the protective effect of the hydrophilic singlet oxygen scavenger ascorbate was maximal for AlPcS3 and minimal for amide of chlorin e6 dimethyl ester. The depth of photosensitizer position in the lipid bilayer was estimated from the quenching of photosensitizer fluorescence by iodide. Thus, the protective effect of a singlet oxygen scavenger against photodynamic inactivation of the membrane-inserted peptide is enhanced upon location of the photosensitizer and scavenger molecules in close vicinity to each other.
- Subjects :
- Ascorbic Acid pharmacology
Gramicidin metabolism
Hydrophobic and Hydrophilic Interactions
Indoles chemistry
Ion Channels metabolism
Lipid Bilayers metabolism
Organometallic Compounds chemistry
Photochemistry
Porphyrins chemistry
Porphyrins metabolism
Singlet Oxygen chemistry
Singlet Oxygen metabolism
Gramicidin chemistry
Ion Channels chemistry
Lipid Bilayers chemistry
Photosensitizing Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1608-3040
- Volume :
- 80
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Biochemistry. Biokhimiia
- Publication Type :
- Academic Journal
- Accession number :
- 26531019
- Full Text :
- https://doi.org/10.1134/S0006297915060097