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A novel insect-specific flavivirus replicates only in Aedes-derived cells and persists at high prevalence in wild Aedes vigilax populations in Sydney, Australia.

Authors :
McLean BJ
Hobson-Peters J
Webb CE
Watterson D
Prow NA
Nguyen HD
Hall-Mendelin S
Warrilow D
Johansen CA
Jansen CC
van den Hurk AF
Beebe NW
Schnettler E
Barnard RT
Hall RA
Source :
Virology [Virology] 2015 Dec; Vol. 486, pp. 272-83. Date of Electronic Publication: 2015 Oct 27.
Publication Year :
2015

Abstract

To date, insect-specific flaviviruses (ISFs) have only been isolated from mosquitoes and increasing evidence suggests that ISFs may affect the transmission of pathogenic flaviviruses. To investigate the diversity and prevalence of ISFs in Australian mosquitoes, samples from various regions were screened for flaviviruses by ELISA and RT-PCR. Thirty-eight pools of Aedes vigilax from Sydney in 2007 yielded isolates of a novel flavivirus, named Parramatta River virus (PaRV). Sequencing of the viral RNA genome revealed it was closely related to Hanko virus with 62.3% nucleotide identity over the open reading frame. PaRV failed to grow in vertebrate cells, with only Aedes-derived mosquito cell lines permissive to replication, suggesting a narrow host range. 2014 collections revealed that PaRV had persisted in A. vigilax populations in Sydney, with 88% of pools positive. Further investigations into its mode of transmission and potential to influence vector competence of A. vigilax for pathogenic viruses are warranted.<br /> (Copyright © 2015. Published by Elsevier Inc.)

Details

Language :
English
ISSN :
1096-0341
Volume :
486
Database :
MEDLINE
Journal :
Virology
Publication Type :
Academic Journal
Accession number :
26519596
Full Text :
https://doi.org/10.1016/j.virol.2015.07.021