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Frequent occurrence of T cell-mediated late reactions revealed by atopy patch testing with hypoallergenic rBet v 1 fragments.
- Source :
-
The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 2016 Feb; Vol. 137 (2), pp. 601-609.e8. Date of Electronic Publication: 2015 Oct 28. - Publication Year :
- 2016
-
Abstract
- Background: Late allergic reactions are common in the course of allergen-specific immunotherapy and even occur with allergy vaccines with reduced IgE reactivity.<br />Objective: We sought to study atopy patch test (APT) reactions and T-cell responses to the recombinant birch pollen allergen Bet v 1 and recombinant hypoallergenic T-cell epitope-containing Bet v 1 fragments in patients with birch pollen allergy with and without atopic dermatitis (AD).<br />Methods: A clinical study was conducted in 15 patients with birch pollen allergy with AD (group 1), 5 patients with birch pollen allergy without AD (group 2), 5 allergic patients without birch pollen allergy (group 3), and 5 nonallergic subjects (group 4) by performing skin prick tests and APTs with rBet v 1 and hypoallergenic rBet v 1 fragments. T-cell, cutaneous lymphocyte antigen (CLA)(+) and CCR4(+) T-cell and cytokine responses were studied by thymidine uptake, carboxyfluorescein diacetate succinimidyl ester staining, and Luminex technology, respectively.<br />Results: rBet v 1 and hypoallergenic rBet v 1 fragments induced APT reactions in not only most of the patients with birch pollen allergy with AD (11/15) but also in most of those without AD (4/5). Patients with birch pollen allergy with AD had higher Bet v 1-specific proliferation of CLA(+) and CCR4(+) T cells compared with patients with birch pollen allergy without AD. There were no differences in Bet v 1-specific CLA(+) and CCR4(+) proliferation and cytokine secretion in patients with and without APT reactions.<br />Conclusion: Hypoallergenic rBet v 1 fragments induce T cell-dependent late reactions not only in patients with birch pollen allergy with AD but also in those without AD, which can be determined based on APT results but not based on in vitro parameters.<br /> (Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Adult
Betula adverse effects
Cytokines biosynthesis
Dermatitis, Atopic diagnosis
Dermatitis, Atopic immunology
Dermatitis, Atopic metabolism
Female
Histamine Release
Humans
Hypersensitivity, Delayed metabolism
Hypersensitivity, Immediate diagnosis
Hypersensitivity, Immediate immunology
Hypersensitivity, Immediate metabolism
Lymphocyte Activation immunology
Male
Pollen immunology
Rhinitis, Allergic, Seasonal diagnosis
Rhinitis, Allergic, Seasonal immunology
T-Cell Antigen Receptor Specificity immunology
T-Lymphocytes metabolism
Young Adult
Allergens immunology
Antigens, Plant immunology
Hypersensitivity, Delayed diagnosis
Hypersensitivity, Delayed immunology
Patch Tests
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-6825
- Volume :
- 137
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of allergy and clinical immunology
- Publication Type :
- Academic Journal
- Accession number :
- 26518092
- Full Text :
- https://doi.org/10.1016/j.jaci.2015.08.042