Back to Search
Start Over
Dental enamel cells express functional SOCE channels.
- Source :
-
Scientific reports [Sci Rep] 2015 Oct 30; Vol. 5, pp. 15803. Date of Electronic Publication: 2015 Oct 30. - Publication Year :
- 2015
-
Abstract
- Dental enamel formation requires large quantities of Ca(2+) yet the mechanisms mediating Ca(2+) dynamics in enamel cells are unclear. Store-operated Ca(2+) entry (SOCE) channels are important Ca(2+) influx mechanisms in many cells. SOCE involves release of Ca(2+) from intracellular pools followed by Ca(2+) entry. The best-characterized SOCE channels are the Ca(2+) release-activated Ca(2+) (CRAC) channels. As patients with mutations in the CRAC channel genes STIM1 and ORAI1 show abnormal enamel mineralization, we hypothesized that CRAC channels might be an important Ca(2+) uptake mechanism in enamel cells. Investigating primary murine enamel cells, we found that key components of CRAC channels (ORAI1, ORAI2, ORAI3, STIM1, STIM2) were expressed and most abundant during the maturation stage of enamel development. Furthermore, inositol 1,4,5-trisphosphate receptor (IP3R) but not ryanodine receptor (RyR) expression was high in enamel cells suggesting that IP3Rs are the main ER Ca(2+) release mechanism. Passive depletion of ER Ca(2+) stores with thapsigargin resulted in a significant raise in [Ca(2+)]i consistent with SOCE. In cells pre-treated with the CRAC channel blocker Synta-66 Ca(2+) entry was significantly inhibited. These data demonstrate that enamel cells have SOCE mediated by CRAC channels and implicate them as a mechanism for Ca(2+) uptake in enamel formation.
- Subjects :
- Ameloblasts metabolism
Ameloblasts pathology
Animals
Calcium Channel Blockers pharmacology
Calcium Channels chemistry
Calcium Channels genetics
Calcium Signaling drug effects
Calcium-Binding Proteins chemistry
Calcium-Binding Proteins genetics
Calcium-Binding Proteins metabolism
Cells, Cultured
Dental Enamel cytology
Fura-2 chemistry
Fura-2 metabolism
Humans
Inositol 1,4,5-Trisphosphate Receptors genetics
Inositol 1,4,5-Trisphosphate Receptors metabolism
Male
Membrane Glycoproteins chemistry
Membrane Glycoproteins genetics
Membrane Glycoproteins metabolism
Membrane Proteins chemistry
Membrane Proteins genetics
Membrane Proteins metabolism
Mice
Mice, Inbred C57BL
ORAI1 Protein
Rats
Rats, Sprague-Dawley
Ryanodine Receptor Calcium Release Channel genetics
Ryanodine Receptor Calcium Release Channel metabolism
Sarcoplasmic Reticulum Calcium-Transporting ATPases genetics
Sarcoplasmic Reticulum Calcium-Transporting ATPases metabolism
Stromal Interaction Molecule 1
Stromal Interaction Molecule 2
Thapsigargin pharmacology
Calcium Channels metabolism
Dental Enamel metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 5
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 26515404
- Full Text :
- https://doi.org/10.1038/srep15803