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Effect of tumor necrosis factor and granulocyte/macrophage colony-stimulating factor on neutrophil degranulation.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1989 May 01; Vol. 142 (9), pp. 3199-205. - Publication Year :
- 1989
-
Abstract
- Both TNF and and granulocyte/macrophage CSF (GM-CSF) can activate neutrophils. The aim of this work was to determine the effect of these cytokines on neutrophil degranulation. The secretion of lactoferrin of secondary granules and myeloperoxidase (MPO) of primary granules from single adherent human neutrophils was assayed by use of a reverse hemolytic plaque assay. Both rTNF and rGM-CSF caused secretion of lactoferrin in a dose-dependent manner. Both agents also caused secretion of MPO, but only in the presence of cytochalasin B. Preincubation with pertussis toxin inhibited rGM-CSF-induced secretion of both lactoferrin and MPO. rTNF-induced MPO secretion was also blocked by pertussis toxin, whereas lactoferrin secretion was only slightly affected. Neither rTNF nor rGM-CSF caused any detectable changes in the concentration of cytoplasmic free Ca2+ in fura-2-loaded cells. However, when neutrophils were loaded with increasing concentrations of quin-2 to buffer any local, not detectable, changes in the concentration of cytoplasmic Ca2+, both rTNF- and rGM-CSF-induced secretion of lactoferrin and MPO were almost totally abolished at a relatively low quin-2 concentration. These results suggest a role of a regulatory G-protein and minute local changes in the concentration of cytoplasmic Ca2+ in TNF- and GM-CSF-induced neutrophil degranulation.
- Subjects :
- Buffers
Calcium metabolism
Calcium physiology
Cytosol metabolism
Exocytosis
Granulocyte-Macrophage Colony-Stimulating Factor
Humans
Neutrophils physiology
Pertussis Toxin
Signal Transduction drug effects
Virulence Factors, Bordetella pharmacology
Colony-Stimulating Factors pharmacology
Cytoplasmic Granules metabolism
Growth Substances pharmacology
Neutrophils metabolism
Tumor Necrosis Factor-alpha pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 142
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 2651522