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Identification of alanyl aminopeptidase (CD13) as a surface marker for isolation of mature gastric zymogenic chief cells.
- Source :
-
American journal of physiology. Gastrointestinal and liver physiology [Am J Physiol Gastrointest Liver Physiol] 2015 Dec 15; Vol. 309 (12), pp. G955-64. Date of Electronic Publication: 2015 Oct 29. - Publication Year :
- 2015
-
Abstract
- Injury and inflammation in the gastric epithelium can cause disruption of the pathways that guide the differentiation of cell lineages, which in turn can cause persistent alterations in differentiation patterns, known as metaplasia. Metaplasia that occurs in the stomach is associated with increased risk for cancer. Methods for isolating distinct gastric epithelial cell populations would facilitate dissection of the molecular and cellular pathways that guide normal and metaplastic differentiation. Here, we identify alanyl aminopeptidase (CD13) as a specific surface marker of zymogenic chief cells (ZCs) in the gastric epithelium. We show that 1) among gastric epithelial cells alanyl aminopeptidase expression is confined to mature ZCs, and 2) its expression is lost en route to metaplasia in both mouse and human stomachs. With this new marker coupled with new techniques that we introduce for dissociating gastric epithelial cells and overcoming their constitutive autofluorescence, we are able to reliably isolate enriched populations of ZCs for both molecular analysis and for the establishment of ZC-derived ex vivo gastroid cultures.<br /> (Copyright © 2015 the American Physiological Society.)
- Subjects :
- Adaptor Proteins, Signal Transducing
Animals
Biomarkers metabolism
Carrier Proteins genetics
Carrier Proteins metabolism
Cells, Cultured
Chief Cells, Gastric pathology
Female
Humans
Male
Metaplasia
Mice, Inbred C57BL
Mice, Knockout
Phenotype
Stomach pathology
CD13 Antigens metabolism
Cell Separation methods
Chief Cells, Gastric enzymology
Stomach enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1547
- Volume :
- 309
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Gastrointestinal and liver physiology
- Publication Type :
- Academic Journal
- Accession number :
- 26514774
- Full Text :
- https://doi.org/10.1152/ajpgi.00261.2015