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Mycoplasma Infection Alters Cancer Stem Cell Properties in Vitro.
- Source :
-
Stem cell reviews and reports [Stem Cell Rev Rep] 2016 Feb; Vol. 12 (1), pp. 156-61. - Publication Year :
- 2016
-
Abstract
- Cancer cell lines can be useful to model cancer stem cells. Infection with Mycoplasma species is an insidious problem in mammalian cell culture. While investigating stem-like properties in early passage melanoma cell lines, we noted poorly reproducible results from an aliquot of a cell line that was later found to be infected with Mycoplasma hyorhinis. Deliberate infection of other early passage melanoma cell lines aliquots induced variable and unpredictable effects on expression of putative cancer stem cell markers, clonogenicity, proliferation and global gene expression. Cell lines established in stem cell media (SCM) were equally susceptible. Mycoplasma status is rarely reported in publications using cultured cells to study the cancer stem cell hypothesis. Our work highlights the importance of surveillance for Mycoplasma infection while using any cultured cells to interrogate tumor heterogeneity.
- Subjects :
- Bacterial Typing Techniques
Cell Culture Techniques standards
Cell Line, Tumor
Host-Pathogen Interactions
Humans
Melanoma genetics
Melanoma metabolism
Melanoma microbiology
Melanoma pathology
Mycoplasma hyorhinis genetics
Neoplasm Proteins metabolism
Neoplastic Stem Cells metabolism
Polymerase Chain Reaction
Primary Cell Culture
Quality Control
RNA, Ribosomal, 16S genetics
Reproducibility of Results
Skin Neoplasms genetics
Skin Neoplasms metabolism
Skin Neoplasms microbiology
Skin Neoplasms pathology
Gene Expression Regulation, Neoplastic
Mycoplasma hyorhinis isolation & purification
Neoplasm Proteins genetics
Neoplastic Stem Cells microbiology
RNA, Ribosomal, 16S isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 2629-3277
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Stem cell reviews and reports
- Publication Type :
- Academic Journal
- Accession number :
- 26514153
- Full Text :
- https://doi.org/10.1007/s12015-015-9630-8