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Sequencing and de novo draft assemblies of a fathead minnow (Pimephales promelas) reference genome.
- Source :
-
Environmental toxicology and chemistry [Environ Toxicol Chem] 2016 Jan; Vol. 35 (1), pp. 212-7. Date of Electronic Publication: 2015 Oct 29. - Publication Year :
- 2016
-
Abstract
- The present study was undertaken to provide the foundation for development of genome-scale resources for the fathead minnow (Pimephales promelas), an important model organism widely used in both aquatic toxicology research and regulatory testing. The authors report on the first sequencing and 2 draft assemblies for the reference genome of this species. Approximately 120× sequence coverage was achieved via Illumina sequencing of a combination of paired-end, mate-pair, and fosmid libraries. Evaluation and comparison of these assemblies demonstrate that they are of sufficient quality to be useful for genome-enabled studies, with 418 of 458 (91%) conserved eukaryotic genes mapping to at least 1 of the assemblies. In addition to its immediate utility, the present work provides a strong foundation on which to build further refinements of a reference genome for the fathead minnow.<br /> (© 2015 SETAC.)
Details
- Language :
- English
- ISSN :
- 1552-8618
- Volume :
- 35
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Environmental toxicology and chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 26513338
- Full Text :
- https://doi.org/10.1002/etc.3186