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[Fish growth-hormone genes: functionality evidence of paralogous genes in Levanidov's charr].
- Source :
-
Molekuliarnaia biologiia [Mol Biol (Mosk)] 2015 Sep-Oct; Vol. 49 (5), pp. 770-6. - Publication Year :
- 2015
-
Abstract
- In the genome of most vertebrates growth-hormone gene is presented in a single copy, while in salmonids after one of the duplication events many genes were multiplied, including growth hormone gene. In salmonids, the growth-hormone gene exists as two independently inherited functional paralogues, gh1 and gh2. In this study, we performed a comparative analysis of gh1 and gh2 growth-hormone genes and their adjacent sequences in Levanidov's charr Salvelinus levanidovi to determine their functionality and define the potential differences. We found that both genes have the same gene structure and are composed of six exons (I-VI) and five introns (A, B, C, D, E). However, the respective gene sequences differ in length. A comparison of exons showed that the size of each exon is identical in both paralogues. The overall length of genes differs due to the varying lengths of introns. Coding sequence of both genes contains an open reading frame for 210 amino acids. We identified regulatory elements in the promoter region of both genes: TATA box, A/T-rich regions that contain binding sites for pituitary-specific transcriptional activator Pit-1, and regions responsible for interaction with other transcriptional activators and initiators, in particular hormone receptors. The obtained data indicate that both genes are functional.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Binding Sites
Escherichia coli genetics
Escherichia coli metabolism
Exons
Fish Proteins chemistry
Fish Proteins metabolism
Gene Expression
Growth Hormone chemistry
Growth Hormone metabolism
Introns
Molecular Sequence Data
Protein Binding
Protein Isoforms chemistry
Protein Isoforms genetics
Protein Isoforms metabolism
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins metabolism
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Transcription Factor Pit-1 genetics
Transcription Factor Pit-1 metabolism
Trout metabolism
Fish Proteins genetics
Growth Hormone genetics
Open Reading Frames
Promoter Regions, Genetic
Trout genetics
Subjects
Details
- Language :
- Russian
- ISSN :
- 0026-8984
- Volume :
- 49
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Molekuliarnaia biologiia
- Publication Type :
- Academic Journal
- Accession number :
- 26510594
- Full Text :
- https://doi.org/10.7868/S0026898415050092