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Surface air plasma-induced cell death and cytokine release of human keratinocytes in the context of psoriasis.
- Source :
-
The British journal of dermatology [Br J Dermatol] 2016 Mar; Vol. 174 (3), pp. 542-52. Date of Electronic Publication: 2015 Dec 30. - Publication Year :
- 2016
-
Abstract
- Background: Cold atmospheric plasma (CAP) has shown promise for wound healing, although little is understood of the underpinning mechanisms. Little has been reported so far of its potential use in the treatment of immune-mediated diseases such as psoriasis.<br />Objectives: To study CAP-induced cell death and cytokine release in human keratinocytes as a first assessment of possible CAP use for psoriasis.<br />Methods: Using a CAP generator free of energetic ions, we observed its effects on keratinocytes in terms of morphology, cell viability and apoptosis, intracellular and mitochondrial reactive oxygen species (ROS), lysosomal integrity and mitochondrial membrane potential; and on secretion and expression of eight cytokines at protein and gene levels.<br />Results: CAP-induced reduced cell viability, apoptotic death and production of intracellular and mitochondrial ROS in dose-dependent manner. Mitochondrial dysfunction and lysosomal leakage were found in CAP-treated cells. It also induced release of interleukin (IL)-6, IL-8, tumour necrosis factor (TNF)-α and vascular endothelial growth factor (VEGF), and enhanced the mRNA expression of IL-1β, IL-6, IL-8, IL-10, TNF-α, interferon-γ and VEGF. By contrast, IL-12 declined monotonically.<br />Conclusions: The results suggest that with appropriate control of its dose, physical plasma could induce cell death via apoptotic pathways and enable simultaneous reduction in IL-12. These effects may be used to suppress keratinocyte hyperproliferation and to target T-cell activation to control amplification of inflammation. This provides an initial basis for further studies of CAP as a potential therapeutic option for inflammatory and immune-related diseases in dermatology, including psoriasis.<br /> (© 2015 British Association of Dermatologists.)
- Subjects :
- Caspase 3 metabolism
Caspase 9 metabolism
Cell Survival physiology
Cells, Cultured
Humans
Hydrogen Peroxide metabolism
Keratinocytes pathology
Lysosomes physiology
Membrane Potential, Mitochondrial physiology
Mitochondria chemistry
Nitrates metabolism
Nitrites metabolism
RNA, Messenger metabolism
Reactive Oxygen Species metabolism
Cell Death physiology
Cytokines biosynthesis
Keratinocytes metabolism
Plasma
Psoriasis therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1365-2133
- Volume :
- 174
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The British journal of dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 26498849
- Full Text :
- https://doi.org/10.1111/bjd.14236