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The MRTF-A/B function as oncogenes in pancreatic cancer.
- Source :
-
Oncology reports [Oncol Rep] 2016 Jan; Vol. 35 (1), pp. 127-38. Date of Electronic Publication: 2015 Oct 15. - Publication Year :
- 2016
-
Abstract
- Despite evidence that MRTF-A/B, co-activators of serum response factor (SRF), promotes tumor cell invasion and metastasis in cancer, there are no studies describing MRTF-A/B in pancreatic cancer. To clarify involvement of MRTF-A/B expression in pancreatic cancer, we used quantitative reverse transcription-polymerase chain reaction and western blot analysis to detect MRTF-A/B in pancreatic cancer, intraductal papillary mucinous neoplasm (IPMN) and non-neoplastic pancreata. MRTF-A/B expression differs significantly between cancer and non-neoplastic tissues as well as between non-neoplastic tissues and IPMN bulk tissues. Next, we studied the roles of MRTF-A/B in vitro. Overexpression of MRTF-A/B promoted epithelial-mesenchymal transition (EMT) and generated stem cell-like cells in normal pancreatic cells. We performed quantitative reverse transcription-polymerase chain reaction to detect the level of MRTF-A/B in 19 pancreatic cancer cell lines. We found that their expression was associated with gemcitabine resistance. Like in normal pancreatic cells, MRTF-A/B also promoted EMT and promoted formation of stem cell-like cells in pancreatic cancer and they could regulate microRNA expression associated with EMT and CICs. Finally, to further demonstrate the roles of MRTF-A/B in vivo, we performed nude mouse model of s.c. xenograft and found that overexpression of MRTF-A and MRTF-B promoted pancreatic cancer growth. Elucidating the roles of MRTF-A/B will help us to further understand molecular basis of the disease and offer new gene targets for effective therapies.
- Subjects :
- Animals
Cell Line, Tumor
Deoxycytidine analogs & derivatives
Deoxycytidine pharmacology
Drug Resistance, Neoplasm
Epithelial-Mesenchymal Transition
Humans
Mice
Neoplasm Transplantation
Neoplastic Stem Cells metabolism
Neoplastic Stem Cells pathology
Pancreatic Neoplasms metabolism
Pancreatic Neoplasms pathology
Trans-Activators metabolism
Transcription Factors metabolism
Gemcitabine
Gene Expression Regulation, Neoplastic
Pancreatic Neoplasms genetics
Trans-Activators genetics
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2431
- Volume :
- 35
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 26498848
- Full Text :
- https://doi.org/10.3892/or.2015.4329