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Evaluation of enzyme immobilization methods for paper-based devices--A glucose oxidase study.
- Source :
-
Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2016 Jan 05; Vol. 117, pp. 551-9. Date of Electronic Publication: 2015 Oct 01. - Publication Year :
- 2016
-
Abstract
- Paper-based sensors gained almost explosive attention during the last few years. A large number of systems, often destined to resource limited settings is based on enzymatic reactions. Choice of an adequate immobilization method could significantly prolong the shelf-life of such sensors, especially in applications, where exposure to high temperatures during storage and transport is more than a threat. We are seeking to compare a variety of immobilization methods based on different phenomena (adsorption, entrapment in gel, microencapsulation, covalent linkage), with total of 33 methods tested. Glucose oxidase was used as a model enzyme. Enzymatic activity of immobilized samples was accompanied for a period of 24 weeks considering two sets of samples, one stored in 4 °C and other in ambient temperature.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-264X
- Volume :
- 117
- Database :
- MEDLINE
- Journal :
- Journal of pharmaceutical and biomedical analysis
- Publication Type :
- Academic Journal
- Accession number :
- 26498392
- Full Text :
- https://doi.org/10.1016/j.jpba.2015.08.041