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Effect of equine herpesvirus type 1 (EHV-1) infection of nasal mucosa epithelial cells on integrin alpha 6 and on different components of the basement membrane.
- Source :
-
Archives of virology [Arch Virol] 2016 Jan; Vol. 161 (1), pp. 103-10. Date of Electronic Publication: 2015 Oct 23. - Publication Year :
- 2016
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Abstract
- The respiratory mucosa is the common port of entry of equine herpesvirus type 1 (EHV-1) and several other alphaherpesviruses. An important prerequisite for successful host invasion of the virus is to cross the epithelial cell layer and the underlying basement membrane barrier. In the present study, an analysis was performed to see if an EHV-1 infection of nasal mucosa epithelial cells leads to damage of the underlying extracellular matrix proteins. Nasal mucosa explants were inoculated with EHV-1 and collected at 0, 24 and 48 hours post-inoculation (hpi). Then, double immunofluorescence staining was performed to detect viral-antigen-positive cells on the one hand and integrin alpha 6, laminin, collagen IV and collagen VII on the other hand. The area of these extracellular matrix proteins was measured in regions of interest (ROIs) at a magnification of 200X by means of the software imaging system ImageJ. ROIs were defined beneath uninfected and infected regions. In uninfected regions, 22-28 % of the ROI was stained for integrin alpha 6, 18-37 % for laminin, 14-38 % for collagen IV and 18-26 % for collagen VII. In infected regions, the percentage positive for integrin alpha 6 was significantly decreased to 0.1-9 % and 0.1-6 % after 24 and 48 hours of inoculation, respectively. Infection did not alter the percentages for laminin and collagen IV. For collagen VII, an increase in the percentage (from 18-26 % to 28-39 %) could be observed underneath EHV-1-infected plaques at 48 hours of inoculation. In conclusion, the results revealed a substantial impact of EHV-1 infection on integrin alpha 6 and collagen VII, two important components of the extracellular matrix, which are associated with the basement membrane and may facilitate virus penetration via hijacked leukocytes to underlying tissues.
- Subjects :
- Animals
Basement Membrane virology
Epithelial Cells virology
Herpesviridae Infections genetics
Herpesviridae Infections metabolism
Herpesviridae Infections virology
Herpesvirus 1, Equid genetics
Horse Diseases genetics
Horse Diseases virology
Horses
Integrin alpha6 genetics
Nasal Mucosa virology
Basement Membrane metabolism
Epithelial Cells metabolism
Herpesviridae Infections veterinary
Herpesvirus 1, Equid physiology
Horse Diseases metabolism
Integrin alpha6 metabolism
Nasal Mucosa metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-8798
- Volume :
- 161
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 26497179
- Full Text :
- https://doi.org/10.1007/s00705-015-2643-4