Switchable fluorescence of gold nanoclusters for probing the activity of alkaline phosphatase and its application in immunoassay.

Authors :: Hu XL
Wu XM
Fang X
Li ZJ
Wang GL
Source :: Biosensors & bioelectronics [Biosens Bioelectron] 2016 Mar 15; Vol. 77, pp. 666-72. Date of Electronic Publication: 2015 Oct 23.
Publication Year :: 2016
Abstract: In this work, a novel strategy for modulating the fluorescence of gold nanoclusters (Au NCs) is developed. The fluorescence of bovine serum albumin (BSA) protected Au NCs is firstly quenched by KMnO4 and then restored by ascorbic acid (AA) due to the deterioration/restoration of the surface structure. Based on which, a novel "switch-on" fluorescent assay for probing the activity of alkaline phosphatase (ALP) is developed with a detection limit as low as 0.002 U/L. In addition, this testing protocol is also expanded to the detection of the inhibitor of ALP and mouse IgG (as a model), the detection limits are 15 ng/mL for the inhibitor of 2,4-Dichlorophenoxyacetic acid (2,4-DA) and 1.5 pg/mL for mouse IgG. The present method paves a new way to develop convenient, sensitive, and selective metal NCs-based fluorescent "turn-on" probes with promising applications in versatile biosensing.<br /> (Copyright © 2015 Elsevier B.V. All rights reserved.)

Subjects :: Alkaline Phosphatase chemistry
Enzyme Activation
Equipment Design
Equipment Failure Analysis
Metal Nanoparticles ultrastructure
Molecular Probes chemistry
Reproducibility of Results
Sensitivity and Specificity
Alkaline Phosphatase analysis
Gold chemistry
Immunoassay instrumentation
Metal Nanoparticles chemistry
Molecular Probe Techniques instrumentation
Spectrometry, Fluorescence instrumentation

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